Betalain biosynthesis in red pulp pitaya is regulated via HuMYB132: a R-R type MYB transcription factor.
Betalain biosynthesis in red pulp pitaya is regulated via HuMYB132: a R-R type MYB transcription factor.
复制标题
R-R型MYB转录因子HuMYB 132对红肉火龙果β-内酰胺酶生物合成的调控
DOI：
10.1186/s12870-023-04049-6
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发表时间：
2023-01-13
影响因子：
5.3
通讯作者：
Qin, Yonghua
中科院分区：
文献类型：
--
作者：
Xie, Fangfang;Chen, Canbin;Chen, Jiayi;Chen, Jiaxuan;Hua, Qingzhu;Shah, Kamran;Zhang, Zhike;Zhao, Jietang;Hu, Guibing;Chen, Jianye;Qin, Yonghua
Multiple MYB transcription factors (TFs) are involved in the regulation of plant coloring. Betalain is a kind of natural plant pigment and its biosynthesis is regulated by a number of enzymes. Despite this, little is known about the molecular properties and roles of MYB TFs in pitaya betalain biosynthesis. In the present study, we identified a 1R-MYB gene, HuMYB132, which is preferentially expressed in red-pulp pitaya at the mature stage. It was clustered with Arabidopsis R-R-type genes and had two DNA-binding domains and a histidine-rich region. The expression assays in N. benthamiana and yeast indicated that HuMYB132 is a nucleus-localized protein with transcriptional activation activity. Dual luciferase reporter assay and electrophoretic mobility shift assays (EMSA) demonstrated that HuMYB132 could promote the transcriptional activities of HuADH1, HuCYP76AD1–1, and HuDODA1 by binding to their promoters. Silencing HuMYB132 reduced betalain accumulation and the expression levels of betalain biosynthetic genes in pitaya pulps. According to our findings, HuMYB132, a R-R type member of 1R-MYB TF subfamily, positively regulates pitaya betalain biosynthesis by regulating the expression of HuADH1, HuCYP76AD1–1, and HuDODA1. The present study provides a new theoretical reference for the management of pitaya betalain biosynthesis and also provides an essential basis for future regulation of betalain biosynthesis in Hylocereus. The online version contains supplementary material available at 10.1186/s12870-023-04049-6.
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DOI：
10.1111/nph.13441
发表时间：
2015-09
期刊：
The New phytologist
影响因子：
--
作者：
Brockington SF;Yang Y;Gandia-Herrero F;Covshoff S;Hibberd JM;Sage RF;Wong GK;Moore MJ;Smith SA
通讯作者：
Smith SA
CsMYB3 和 CsRuby1 形成“激活剂和阻抑剂”环，用于调节柑橘花青素生物合成
影响因子：
4.9
作者：
Huang, Ding;Tang, Zhouzhou;Xu, Qiang
通讯作者：
Xu, Qiang
影响因子：
8.7
作者：
Chen JY;Xie FF;Cui YZ;Chen CB;Lu WJ;Hu XD;Hua QZ;Zhao J;Wu ZJ;Gao D;Zhang ZK;Jiang WK;Sun QM;Hu GB;Qin YH
通讯作者：
Qin YH
影响因子：
5.6
作者：
Hua Q;Zhou Q;Gan S;Wu J;Chen C;Li J;Ye Y;Zhao J;Hu G;Qin Y
通讯作者：
Qin Y
影响因子：
5.6
作者：
Agrahari RK;Enomoto T;Ito H;Nakano Y;Yanase E;Watanabe T;Sadhukhan A;Iuchi S;Kobayashi M;Panda SK;Yamamoto YY;Koyama H;Kobayashi Y
通讯作者：
Kobayashi Y