Proteomic Analysis of Hylocereus polyrhizus Reveals Metabolic Pathway Changes.
Proteomic Analysis of Hylocereus polyrhizus Reveals Metabolic Pathway Changes.
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DOI：
10.3390/ijms17101606
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发表时间：
2016-09-28
影响因子：
5.6
通讯作者：
Qin Y
中科院分区：
文献类型：
--
作者：
Hua Q;Zhou Q;Gan S;Wu J;Chen C;Li J;Ye Y;Zhao J;Hu G;Qin Y
红火龙果或红火龙果（Hylocereus polyrhizus）是唯一含有甜菜素的可食用水果。甜菜素在植物中的颜色从红色、紫色到黄色不等。Betalains也可以作为促进健康和预防疾病的功能食品的重要组成部分。目前，甜菜素生产的生物合成和调控途径仍有待完全破译。本研究采用等压标签相对定量和绝对定量（iTRAQ）技术对多根麻果实进行蛋白质组学分析，揭示了白浆期和红浆期甜菜素生物合成的分子机制。鉴定出1946个差异表达蛋白，其中936个蛋白在多根水蛭红浆期显著高表达。RNA-seq和iTRAQ分析显示，部分转录本与蛋白呈正相关；它们分别属于“苯丙素生物合成”、“酪氨酸代谢”、“类黄酮生物合成”、“抗坏血酸和醛酸代谢”、“甜菜碱生物合成”和“花青素生物合成”。在甜菜素生物合成途径中，鉴定出了多酚氧化酶、CYP76AD3和4,5-二羟基苯基丙氨酸（DOPA）双加氧酶外二醇样蛋白等多种蛋白/酶。本研究为甜菜素在转录后水平的生物合成提供了新的分子机制。
Red dragon fruit or red pitaya (Hylocereus polyrhizus) is the only edible fruit that contains betalains. The color of betalains ranges from red and violet to yellow in plants. Betalains may also serve as an important component of health-promoting and disease-preventing functional food. Currently, the biosynthetic and regulatory pathways for betalain production remain to be fully deciphered. In this study, isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analyses were used to reveal the molecular mechanism of betalain biosynthesis in H. polyrhizus fruits at white and red pulp stages, respectively. A total of 1946 proteins were identified as the differentially expressed between the two samples, and 936 of them were significantly highly expressed at the red pulp stage of H. polyrhizus. RNA-seq and iTRAQ analyses showed that some transcripts and proteins were positively correlated; they belonged to “phenylpropanoid biosynthesis”, “tyrosine metabolism”, “flavonoid biosynthesis”, “ascorbate and aldarate metabolism”, “betalains biosynthesis” and “anthocyanin biosynthesis”. In betalains biosynthesis pathway, several proteins/enzymes such as polyphenol oxidase, CYP76AD3 and 4,5-dihydroxy-phenylalanine (DOPA) dioxygenase extradiol-like protein were identified. The present study provides a new insight into the molecular mechanism of the betalain biosynthesis at the posttranscriptional level.
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DOI：
10.1111/nph.13441
发表时间：
2015-09
期刊：
The New phytologist
影响因子：
--
作者：
Brockington SF;Yang Y;Gandia-Herrero F;Covshoff S;Hibberd JM;Sage RF;Wong GK;Moore MJ;Smith SA
通讯作者：
Smith SA
影响因子：
3.8
作者：
Isayenkova, Judith;Wray, Victor;Vogt, Thomas
通讯作者：
Vogt, Thomas
影响因子：
5.9
作者：
Clifford T;Howatson G;West DJ;Stevenson EJ
通讯作者：
Stevenson EJ
影响因子：
2.9
作者：
Adnan, Liana;Osman, Azizah;Hamid, Azizah Abdul
通讯作者：
Hamid, Azizah Abdul
影响因子：
4.2
作者：
Allegra, Mario;Ianaro, Angela;Livrea, Maria Antonia
通讯作者：
Livrea, Maria Antonia