High resolution, on-line identification of strains from the Mycobacterium tuberculosis complex based on tandem repeat typing.
High resolution, on-line identification of strains from the Mycobacterium tuberculosis complex based on tandem repeat typing.
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DOI：
10.1186/1471-2180-2-37
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发表时间：
2002-11-27
期刊：
影响因子：
4.2
通讯作者：
Vergnaud G
中科院分区：
文献类型：
--
作者：
Le Flèche P;Fabre M;Denoeud F;Koeck JL;Vergnaud G
目前可用的结核分枝杆菌复合体分子流行病学的参考方法要么缺乏灵敏度，要么对于常规应用来说仍然太繁琐和缓慢。最近，串联重复分型已成为一种潜在的替代方法。本研究为M.通过总结现有的数据，开发额外的标记物，并建立一个免费访问，快速，易于使用，基于互联网的菌株鉴定服务，来研究结核病。采用21个VNTR，包括13个先前描述的基因座和8个新评价的标记，对90株M.结核复合体（M.结核分枝杆菌64株; bovis（9株，包括4株BCG代表株）、M. africanum（17株））。定义了84种不同的基因型。聚类分析表明，M. Africanum菌株分为三大类，其中一类与M.结核菌株，另一个与M.牛品系。由此产生的数据可通过互联网免费访问，以允许直接进行菌株鉴定查询。串联重复序列分型是一种基于PCR的检测方法，它可能被证明是对现有M.肺结核综合征要键入的标记的数量取决于所需的识别精度，使得可以在消耗品、技术专长和设备方面以低成本快速实现识别。
Currently available reference methods for the molecular epidemiology of the Mycobacterium tuberculosis complex either lack sensitivity or are still too tedious and slow for routine application. Recently, tandem repeat typing has emerged as a potential alternative. This report contributes to the development of tandem repeat typing for M. tuberculosis by summarising the existing data, developing additional markers, and setting up a freely accessible, fast, and easy to use, internet-based service for strain identification. A collection of 21 VNTRs incorporating 13 previously described loci and 8 newly evaluated markers was used to genotype 90 strains from the M. tuberculosis complex (M. tuberculosis (64 strains), M. bovis (9 strains including 4 BCG representatives), M. africanum (17 strains)). Eighty-four different genotypes are defined. Clustering analysis shows that the M. africanum strains fall into three main groups, one of which is closer to the M. tuberculosis strains, and an other one is closer to the M. bovis strains. The resulting data has been made freely accessible over the internet to allow direct strain identification queries. Tandem-repeat typing is a PCR-based assay which may prove to be a powerful complement to the existing epidemiological tools for the M. tuberculosis complex. The number of markers to type depends on the identification precision which is required, so that identification can be achieved quickly at low cost in terms of consumables, technical expertise and equipment.
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DOI：
10.1099/00221287-144-5-1189
发表时间：
1998-05-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Frothingham, R;Meeker-O'Connell, WA
通讯作者：
Meeker-O'Connell, WA
影响因子：
9.4
作者：
VANSOOLINGEN, D;QIAN, LS;VANEMBDEN, JDA
通讯作者：
VANEMBDEN, JDA
DOI：
10.1073/pnas.98.4.1901
发表时间：
2001-02-13
影响因子：
11.1
作者：
Mazars, E;Lesjean, S;Supply, P
通讯作者：
Supply, P
影响因子：
9.4
作者：
Supply, P;Lesjean, S;Locht, C
通讯作者：
Locht, C
影响因子：
15.9
作者：
Bayliss, CD;Field, D;Moxon, ER
通讯作者：
Moxon, ER