Molecular cloning and characterization of cystatin, a cysteine protease inhibitor, from Angiostrongylus cantonensis
Molecular cloning and characterization of cystatin, a cysteine protease inhibitor, from Angiostrongylus cantonensis
复制标题
广州管圆线虫半胱氨酸蛋白酶抑制剂半胱氨酸蛋白酶抑制剂的分子克隆和表征
DOI：
10.1007/s00436-010-1952-5
复制
发表时间：
2010-06
影响因子：
2
通讯作者：
Liu, Yu-hong
中科院分区：
文献类型：
--
作者：
Zheng, Huan-qin;Wei, Jie;Han, Yan-ping;Lv, Zhi-yue;Li, Zheng-yu;He, Han-jiang;Xu, Chang-zhi;Zhan, Xi-mei;Wu, Zhong-dao;Liu, Yu-hong
半胱氨酸蛋白酶抑制剂（Cystatins）是在哺乳动物体内普遍存在的巯基蛋白酶抑制剂，并具有多种重要的生理功能。在本研究中，从广州管圆线虫的cDNA文库中克隆出一种新的半胱氨酸蛋白酶抑制剂分子（AcCystatin）
Cystatins are thiol proteinase inhibitors ubiquitously present in mammalian body and serve various important physiological functions. In the present study, a novel cystatin molecule (AcCystatin) was cloned from a cDNA library of Angiostrongylus cantonensis fourth-stage larvae. The putative 14-kDa protein contained 120 residues with cystatin-conserved motifs known to interact with the active site of cysteine peptidases and showed high identities with cystatins from other nematodes. RT-PCR analysis revealed that the expression pattern of AcCystatin was equal at the time points of third-stage larvae, fourth-stage larvae, and adults of the parasite life cycle. The recombinant AcCystatin (rAcCystatin) expressed and purified from Escherichia coli has been demonstrated to possess an obvious inhibitory activity against cathepsin B and could significantly upregulate nitric oxide production from IFN-gamma activated RAW 264.7 macrophages. Sera from mice (non-permissive host) infected with A. cantonensis detected rAcCystatin by Western blot, while the sera from infected rats (permissive host) could not. The results implied that AcCystatin might be an immunoregulator in A. cantonensis infection.
登录
查看更多内容
影响因子：
15.9
作者：
Sukhova, GK;Zhang, Y;Shi, GP
通讯作者：
Shi, GP
影响因子：
2.2
作者：
KANAZAWA, T;ASAHI, H;STADECKER, MJ
通讯作者：
STADECKER, MJ
DOI：
10.1172/jci14915
发表时间：
2003-03
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
G. Sukhova;Yaou Zhang;Jie Pan;Y. Wada;Takashi Yamamoto;M. Naito;T. Kodama;S. Tsimikas;J. Witztum
通讯作者：
G. Sukhova;Yaou Zhang;Jie Pan;Y. Wada;Takashi Yamamoto;M. Naito;T. Kodama;S. Tsimikas;J. Witztum
影响因子：
14.9
作者：
G. Joshua;F. Perler;C. Wang
通讯作者：
G. Joshua;F. Perler;C. Wang
影响因子：
3.8
作者：
Kolodziej, Herbert;Radtke, Oliver A.;Kiderlen, Albrecht F.
通讯作者：
Kiderlen, Albrecht F.