Far-Red Photoactivatable BODIPYs for the Super-Resolution Imaging of Live Cells.
Far-Red Photoactivatable BODIPYs for the Super-Resolution Imaging of Live Cells.
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DOI：
10.1021/jacs.8b09099
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发表时间：
2018-10-10
影响因子：
15
通讯作者：
Raymo, Francisco M.
中科院分区：
文献类型：
--
作者：
Zhang, Yang;Song, Ki-Hee;Tang, Sicheng;Ravelo, Laura;Cusido, Janet;Sun, Cheng;Zhang, Hao F.;Raymo, Francisco M.
带有苯乙烯附体的恶嗪杂环与硼二吡啶（BODIPY）发色团的光诱导断开，激活明亮的远红色荧光。光化学产物的高亮度加上在该光谱区域缺乏任何显著的自身荧光，即使在活细胞的细胞内环境中也可以在单分子水平上进行检测。事实上，这些可光激活的荧光团定位于溶酶体腔室中，当被活性酯功能化时，它们在这些细胞器内保持共价固定。任何显著扩散的抑制允许重复重建图像与亚衍射分辨率和可视化标记的细胞内区室在活细胞中具有优异的定位精度。因此，设计成这些光激活荧光团的光化学，光物理和结构特性的结合转化为在纳米水平上以空间分辨率可视化活细胞的机会，这是传统荧光成像方案无法实现的。
The photoinduced disconnection of an oxazine heterocycle from a borondipyrromethene (BODIPY) chromophore, equipped with a styryl appendage, activates bright far-red fluorescence. The high brightness of the photochemical product together with the lack of any significant autofluorescence in this spectral region allows detection at the single-molecule level even within the intracellular environment of live cells. Indeed, these photoactivatable fluorophores localize in lysosomal compartments and, when functionalized with an active ester, remain covalently immobilized within these organelles. The suppression of any significant diffusion allows the reiterative reconstruction of images with sub-diffraction resolution and the visualization of the labeled intracellular compartments with excellent localization precision in live cells. Thus, the combination of photochemical, photophysical and structural properties designed into these photoactivatable fluorophores translate into the opportunity to visualize live cells with spatial resolution at the nanometer level that is inaccessible to conventional fluorescence imaging schemes.
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