Cilioplasm is a cellular compartment for calcium signaling in response to mechanical and chemical stimuli.
Cilioplasm is a cellular compartment for calcium signaling in response to mechanical and chemical stimuli.
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DOI：
10.1007/s00018-013-1483-1
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发表时间：
2014-06
影响因子：
8
通讯作者：
Nauli, Surya M.
中科院分区：
文献类型：
--
作者：
Jin, Xingjian;Mohieldin, Ashraf M.;Muntean, Brian S.;Green, Jill A.;Shah, Jagesh V.;Mykytyn, Kirk;Nauli, Surya M.
初生纤毛直径约200 nm，与发育和疾病有关。初级纤毛内的钙信号从未被直接观察到，因此一直是一种推测。流体切应力和多巴胺受体5(DR5)激动剂是为数不多的需要纤毛进行细胞内钙信号转导的刺激之一。然而，目前尚不清楚这些刺激是否在纤毛内启动钙信号，或者钙信号是否起源于细胞质。利用集成的单细胞成像技术，我们首次证明了由流体剪切力触发的钙信号在初级纤毛中启动，并可以通过兰尼定受体与随后的胞浆钙反应区分开来。重要的是，这种流诱导的钙信号依赖于纤毛多囊蛋白-2钙通道。DR5特异性激动剂主要通过纤毛CaV1.2诱导纤毛质钙信号，凝血酶通过IP3受体特异性诱导胞浆钙信号。此外，非特异性钙离子载体可同时触发纤毛和胞质钙反应。我们认为纤毛不仅具有感觉细胞器的功能，还具有钙信号隔室的功能。纤毛依赖的信号可以扩散到细胞质，也可以包含在纤毛质中。我们的研究还提供了第一个模型来理解活细胞纤毛质内的信号。
Primary cilia with a diameter of ~200 nm have been implicated in development and disease. Calcium signaling within a primary cilium has never been directly visualized and has therefore remained a speculation. Fluid-shear stress and dopamine receptor type-5 (DR5) agonist are among the few stimuli that require cilia for intracellular calcium signal transduction. However, it is not known if these stimuli initiate calcium signaling within the cilium, or if the calcium signal originates in the cytoplasm. Using an integrated single-cell imaging technique, we demonstrate for the first time that calcium signaling triggered by fluid-shear stress initiates in the primary cilium and can be distinguished from the subsequent cytosolic calcium response through the ryanodine receptor. Importantly, this flow-induced calcium signaling depends on the ciliary polycystin-2 calcium channel. While DR5-specific agonist induces calcium signaling mainly in the cilioplasm via ciliary CaV1.2, thrombin specifically induces cytosolic calcium signaling through the IP3 receptor. Furthermore, a non-specific calcium ionophore triggers both ciliary and cytosolic calcium responses. We suggest that cilia not only act as sensory organelles but also function as calcium signaling compartments. Cilium-dependent signaling can spread to the cytoplasm or be contained within the cilioplasm. Our study also provides the first model to understand signaling within the cilioplasm of a living cell.
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影响因子：
4
作者：
Nakagawa, Taeko;Yamaguchi, Masayoshi
通讯作者：
Yamaguchi, Masayoshi
DOI：
10.1161/hypertensionaha.111.172080
发表时间：
2011-08
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Abdul-Majeed S;Nauli SM
通讯作者：
Nauli SM
影响因子：
13.6
作者：
Nauli, Surya M.;Rossetti, Sandro;Zhou, Jing
通讯作者：
Zhou, Jing
DOI：
10.1152/ajprenal.1997.272.1.f132
发表时间：
1997-01-01
影响因子：
4.2
作者：
Schwartz, EA;Leonard, ML;Bowser, SS
通讯作者：
Bowser, SS
影响因子：
30.8
作者：
Nauli, SM;Alenghat, FJ;Zhou, J
通讯作者：
Zhou, J