Assembly PCR oligo maker: a tool for designing oligodeoxynucleotides for constructing long DNA molecules for RNA production.
Assembly PCR oligo maker: a tool for designing oligodeoxynucleotides for constructing long DNA molecules for RNA production.
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DOI：
10.1093/nar/gki380
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发表时间：
2005-07-01
影响因子：
14.9
通讯作者：
Johnson PE
中科院分区：
文献类型：
--
作者：
Rydzanicz R;Zhao XS;Johnson PE
我们描述了一个计算机程序，组装PCR寡核苷酸制造商，创建自动化的设计寡脱氧核苷酸的PCR为基础的建设长的DNA分子。该程序可在免费获得，并专门设计用于帮助构建DNA分子，这些DNA分子用于通过T7 RNA聚合酶体外合成产生RNA分子。组装PCR Oligo Maker的输入是待制备的所需DNA序列或RNA序列。如果RNA是输入，程序首先确定产生所需RNA分子所需的DNA序列。然后，该程序确定所需DNA分子的两步组装PCR合成所需的所有寡脱氧核苷酸的序列。输出的寡脱氧核苷酸序列被设计为具有均匀的解链温度，并检查PCR反应所需的所需引发区域之外的重叠区域。该程序的有效性进行了实验验证，通过合成一个191-nt长的DNA分子使用的DNA序列建议的程序。
We describe a computer program, Assembly PCR Oligo Maker, created to automate the design of oligodeoxynucleotides for the PCR-based construction of long DNA molecules. This program is freely available at and has been specifically designed to aid in the construction of DNA molecules that are to be used for the production of RNA molecules by in vitro synthesis with T7 RNA polymerase. The input for Assembly PCR Oligo Maker is either the desired DNA sequence to be made or an RNA sequence. If RNA is the input, the program first determines the DNA sequence necessary to produce the desired RNA molecule. The program then determines the sequences of all the oligodeoxynucleotides necessary for a two-step assembly PCR-based synthesis of the desired DNA molecule. The oligodeoxynucleotide sequences outputted are designed to have a uniform melt temperature and are checked for regions of overlap outside of the desired priming regions necessary for the PCR reaction. The validity of the program was verified experimentally by synthesizing a 191-nt long DNA molecule using the DNA sequences suggested by the program.
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影响因子：
2.9
作者：
Allawi, HT;SantaLucia, J
通讯作者：
SantaLucia, J
影响因子：
14.9
作者：
Hoover, DM;Lubkowski, J
通讯作者：
Lubkowski, J
影响因子：
3.5
作者：
STEMMER, WPC;CRAMERI, A;HEYNEKER, HL
通讯作者：
HEYNEKER, HL
DOI：
10.1073/pnas.95.4.1460
发表时间：
1998-02-17
影响因子：
11.1
作者：
SantaLucia, J
通讯作者：
SantaLucia, J
影响因子：
4.5
作者：
Kieft, JS;Batey, RT
通讯作者：
Batey, RT