Citral Attenuated Intestinal Inflammation Induced by Cronobacter sakazakii in Newborn Mice.
Citral Attenuated Intestinal Inflammation Induced by Cronobacter sakazakii in Newborn Mice.
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柠檬醛可减轻新生小鼠阪崎克罗诺杆菌引起的肠道炎症。
DOI：
10.1089/fpd.2019.2729
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发表时间：
2019-11
影响因子：
2.8
通讯作者：
Xia Xiaodong
中科院分区：
文献类型：
--
作者：
Shi Chao;Jin Tong;Guo Du;Zhang Wenting;Yang Baowei;Su Dongfang;Xia Xiaodong
坏死性小肠结肠炎（NEC）是一种严重的炎症性肠道疾病，死亡率高，最常见于新生儿。坂崎克罗伊氏菌是婴儿配方奶粉中的一种常见污染物，与NEC有关。然而，其在NEC发病机制中的作用尚不清楚，并且仍然没有针对NEC的有效治疗方法。目前，已经研究了天然生物活性产品在预防微生物感染中的有益作用。本研究采用新生小鼠肠道炎症模型，研究了天然生物活性物质柠檬醛对C.坂崎引起的肠道炎症和损伤。结果表明，柠檬醛降低了C. sakazakii细胞，并且柠檬醛处理的小鼠的体重显著高于C.坂崎感染的小鼠。柠檬醛治疗还改善了严重的回肠组织损伤，包括上皮脱落、绒毛破裂和肠上皮细胞凋亡。C.坂崎肠杆菌感染可上调回肠组织中多种炎症相关基因的mRNA转录水平，增加IL-6和TNF-α的产生，并激活NF-κB和MAPK信号通路。柠檬醛治疗减轻了这些炎症反应。C.但柠檬醛抑制肠上皮细胞凋亡和这些半胱天冬酶的激活。这些结果表明，柠檬醛对C。sakazakii诱导的新生小鼠肠道炎症，它可能在未来的C.坂崎相关的感染和疾病。
Necrotizing enterocolitis (NEC) is a serious inflammatory intestinal disorder with a high mortality rate, which occurs most commonly in newborn infants. Cronobacter sakazakii, a common contaminant in infant formula, is associated with NEC. However, its role in NEC pathogenesis is unknown, and there are still no effective treatments for NEC. Currently, natural bioactive products have been investigated for their beneficial effects in preventing microbial infection. In this study, a neonatal mouse intestinal inflammation model was used to examine the protective effects of citral (a natural bioactive product) on C. sakazakii-induced intestinal inflammation and damages. It was shown that citral reduced the number of C. sakazakii cells in ileal tissues, and mice treated with citral had a significantly higher body weight than C. sakazakii-infected mice. Citral treatment also ameliorated serious ileal tissue damages, including epithelial sloughing, villous rupture, and enterocyte apoptosis. C. sakazakii infection upregulated the messenger RNA transcription levels of several inflammation-associated genes, increased production of IL-6 and TNF-α, and activated the NF-κB and MAPK signaling pathways in ileal tissues. Citral treatment mitigated these inflammatory responses. The apoptotic index and activities of caspase 3, 8, and 9 increased in murine ileum after C. sakazakii infection, but citral inhibited both enterocyte apoptosis and activations of these caspase. These findings suggest that citral has protective effects on C. sakazakii-induced intestinal inflammation in newborn mice, and it may play a future role in the management of C. sakazakii-associated infections and diseases.
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