Reliability of transcriptional cycles and the yeast cell-cycle oscillator.
Reliability of transcriptional cycles and the yeast cell-cycle oscillator.
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DOI：
10.1371/journal.pcbi.1000842
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发表时间：
2010-07-08
影响因子：
4.3
通讯作者：
Socolar JE
中科院分区：
文献类型：
--
作者：
Sevim V;Gong X;Socolar JE
最近发表的与酵母细胞周期相关的转录振荡器提供了线索，并提出了关于细胞自主循环过程的机制的问题。与文献中的其他生物和合成振荡网络不同，该网络似乎并不依赖于本构信号或正向自动调节，而是通过在决定其周期的缓慢正反馈环路上稳定传输脉冲来进行操作。我们构建了该网络的连续时间布尔模型，该模型允许通过事件时间的小波动对噪声进行建模，并表明它可以维持稳定的振荡。对更简单网络模型的分析显示了如何安排一些构建块以提供针对波动的稳定性。我们的研究结果表明酵母中的转录振荡器属于一类新的生物振荡器。基因阵列等技术能够获取有关基因表达变异的大量数据，从而揭示控制细胞代谢和发育机制的基因调控网络的结构。我们研究了振荡基因调控网络的模型，最近有人认为该模型在维持酵母细胞周期中发挥着不可或缺的作用。该振荡器与其他已知的生物和合成振荡网络的不同之处在于它似乎依赖于长的正反馈回路。我们证明，某些稳定子网络的存在可以解释该振荡器的鲁棒性和不寻常的架构。我们的建模方法阐明了系统的逻辑结构和更新事件计时的重要性。
A recently published transcriptional oscillator associated with the yeast cell cycle provides clues and raises questions about the mechanisms underlying autonomous cyclic processes in cells. Unlike other biological and synthetic oscillatory networks in the literature, this one does not seem to rely on a constitutive signal or positive auto-regulation, but rather to operate through stable transmission of a pulse on a slow positive feedback loop that determines its period. We construct a continuous-time Boolean model of this network, which permits the modeling of noise through small fluctuations in the timing of events, and show that it can sustain stable oscillations. Analysis of simpler network models shows how a few building blocks can be arranged to provide stability against fluctuations. Our findings suggest that the transcriptional oscillator in yeast belongs to a new class of biological oscillators. Technologies such as gene arrays enable acquisition of large amounts of data on gene expression variations, which reveal the structures of gene regulatory networks that govern the metabolic and developmental machinery in the cell. We study a model of an oscillatory gene regulatory network that has been recently suggested to play an integral role in maintaining the cell cycle in yeast. The oscillator differs from other known biological and synthetic oscillatory networks in that it seems to rely on a long positive feedback loop. We show that the presence of certain stabilizing sub-networks can account for the robustness and the unusual architecture of this oscillator. Our modeling approach elucidates both the logical structure of the system and the importance of the timing of update events.
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影响因子：
64.8
作者：
Alon, U;Surette, MG;Leibler, S
通讯作者：
Leibler, S
DOI：
10.1098/rspb.1995.0153
发表时间：
1995-09-22
影响因子：
4.7
作者：
GOLDBETER, A
通讯作者：
GOLDBETER, A
影响因子：
64.8
作者：
Becskei, A;Serrano, L
通讯作者：
Serrano, L
影响因子：
8.6
作者：
Greil, F;Drossel, B
通讯作者：
Drossel, B
影响因子：
64.8
作者：
Haase, SB;Reed, SI
通讯作者：
Reed, SI