Peptidylprolyl isomerase A (PPIA) as a preferred internal control over GAPDH and beta-actin in quantitative RNA analyses.

Peptidylprolyl isomerase A (PPIA) as a preferred internal control over GAPDH and beta-actin in quantitative RNA analyses.
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在定量 RNA 分析中,肽基脯氨酰异构酶 A (PPIA) 作为 GAPDH 和 β-肌动蛋白的首选内部对照。

DOI:
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发表时间:
2002
期刊:
影响因子:
2.7
通讯作者:
Yong Q. Chen
Yong Q. Chen
中科院分区:
工程技术4区
文献类型:
--
作者:
F. Feroze;I. Berquin;J. Dey;Yong Q. Chen

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在基因表达的所有定量分析中,良好的内部控制是至关重要的。分析了78个样本中肌动蛋白、甘油醛-3-磷酸脱氢酶和肽基丙酰异构酶A的水平(数据来自我们的实验室和http://www.ncbi.nlm.nih.gov/SAGE/).的公开数据库这些文库包括来自大脑、乳腺、结肠、肾脏、卵巢、胰腺、前列腺、皮肤和血管来源的细胞系和组织。在这三个基因中,PPIA基因的水平是最恒定的。因此,我们的研究表明,PPIA是一种比两种最常用的内部控制--β-肌动蛋白或GAPDH更好的内部控制。
A good internal control is critical in all quantitative analyses of gene expression. Levels of bet-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and peptidylprolyl isomerase A (PPIA) were analyzed in 78 samples (data obtained from our laboratory and from a publicly available database at http://www.ncbi.nlm.nih.gov/SAGE/). These libraries included cell lines and tissues from brain, breast, colon, kidney, ovary, pancreas, prostate, skin, and vascular origin. The level of PPIA mRNA is the most constant among the three genes. Hence, our study suggests that PPIA is a better internal control than beta-actin or GAPDH, the two most commonly used internal controls.
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