Peptidylprolyl isomerase A (PPIA) as a preferred internal control over GAPDH and beta-actin in quantitative RNA analyses.
Peptidylprolyl isomerase A (PPIA) as a preferred internal control over GAPDH and beta-actin in quantitative RNA analyses.
复制标题
在定量 RNA 分析中,肽基脯氨酰异构酶 A (PPIA) 作为 GAPDH 和 β-肌动蛋白的首选内部对照。
作者:
F. Feroze;I. Berquin;J. Dey;Yong Q. Chen
A good internal control is critical in all quantitative analyses of gene expression. Levels of bet-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and peptidylprolyl isomerase A (PPIA) were analyzed in 78 samples (data obtained from our laboratory and from a publicly available database at http://www.ncbi.nlm.nih.gov/SAGE/). These libraries included cell lines and tissues from brain, breast, colon, kidney, ovary, pancreas, prostate, skin, and vascular origin. The level of PPIA mRNA is the most constant among the three genes. Hence, our study suggests that PPIA is a better internal control than beta-actin or GAPDH, the two most commonly used internal controls.
影响因子:
2.2
作者:
Swift, Benjamin M. C.;Denton, Emily J.;Mahendran, Sophie A.;Huxley, Jonathan N.;Rees, Catherine E. D.
通讯作者:
Rees, Catherine E. D.
DOI:
10.1006/bbrc.1995.1951
发表时间:
1995-07-06
影响因子:
3.1
作者:
MCNULTY, SE;TOSCANO, WA
通讯作者:
TOSCANO, WA
影响因子:
11.2
作者:
Woloschak,GE;Chang-Liu,CM;Jones,PS;Jones,CA
通讯作者:
Jones,CA