Strengths and weaknesses of a new mouse model of thrombosis induced by inferior vena cava stenosis: communication from the SSC of the ISTH.
Strengths and weaknesses of a new mouse model of thrombosis induced by inferior vena cava stenosis: communication from the SSC of the ISTH.
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DOI：
10.1111/jth.12510
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Mackman N
中科院分区：
文献类型：
--
作者：
Geddings J;Aleman MM;Wolberg A;von Brühl ML;Massberg S;Mackman N
方法在此模型中，对至少 8 周龄、体重 22 g、2、3、6 级的麻醉雄性小鼠进行中线剖腹手术。通过无创性钝性解剖暴露 IVC，并小心地将 IVC 与腹主动脉分离。将垫片放置在暴露的 IVC 顶部，并将非反应性永久狭窄结扎线（7.0 或 8.0 单丝聚丙烯）固定在肾静脉正下方的 IVC 和垫片周围。然后移除垫片，导致狭窄部位 2 处的 IVC 管腔尺寸减少约 90%。在手术过程中避免了对 IVC 壁的伤害。排除下腔静脉有任何出血的小鼠。然后关闭手术部位，让小鼠在适当镇痛的影响下恢复。
MethodsIn this model, a midline laparotomy is performed on anesthetized male mice that are at least 8 weeks of age and 22 g in weight 2, 3, 6. The IVC is exposed by atraumatic blunt dissection, and the IVC is carefully separated from the abdominal aorta. A spacer is placed on top of the exposed IVC, and a non-reactive permanent narrowing ligature (7.0 or 8.0 monofil polypropylene) is secured around both the IVC and spacer directly below the renal veins. The spacer is then removed, resulting in a∼ 90% reduction in IVC lumen size at the stenosis site 2. Injury to the IVC wall is avoided during the procedure. Mice with any bleeding from the IVC are excluded. The surgical site is then closed, and mice are allowed to recover under the influence of appropriate analgesia.
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DOI：
10.1084/jem.20112322
发表时间：
2012-04-09
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
von Brühl ML;Stark K;Steinhart A;Chandraratne S;Konrad I;Lorenz M;Khandoga A;Tirniceriu A;Coletti R;Köllnberger M;Byrne RA;Laitinen I;Walch A;Brill A;Pfeiler S;Manukyan D;Braun S;Lange P;Riegger J;Ware J;Eckart A;Haidari S;Rudelius M;Schulz C;Echtler K;Brinkmann V;Schwaiger M;Preissner KT;Wagner DD;Mackman N;Engelmann B;Massberg S
通讯作者：
Massberg S
影响因子：
15.9
作者：
Mackman, Nigel
通讯作者：
Mackman, Nigel
DOI：
10.1111/j.1538-7836.2011.04544.x
发表时间：
2012-01
期刊：
Journal of thrombosis and haemostasis : JTH
影响因子：
--
作者：
Brill A;Fuchs TA;Savchenko AS;Thomas GM;Martinod K;De Meyer SF;Bhandari AA;Wagner DD
通讯作者：
Wagner DD
DOI：
10.1161/atvbaha.112.252130
发表时间：
2012-08-01
影响因子：
8.7
作者：
Brill, Alexander;Yesilaltay, Ayce;Wagner, Denisa D.
通讯作者：
Wagner, Denisa D.
影响因子：
20.3
作者：
Wang, Jian-Guo;Geddings, Julia E.;Mackman, Nigel
通讯作者：
Mackman, Nigel