Major aldehyde dehydrogenase AldFGH of Gluconacetobacter diazotrophicus is independent of pyrroloquinoline quinone but dependent on molybdopterin for acetic acid fermentation
Major aldehyde dehydrogenase AldFGH of Gluconacetobacter diazotrophicus is independent of pyrroloquinoline quinone but dependent on molybdopterin for acetic acid fermentation
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固氮葡糖醋杆菌的主要醛脱氢酶 AldFGH 不依赖于吡咯喹啉醌，但依赖于钼蝶呤进行乙酸发酵
DOI：
10.1007/s00253-021-11144-x
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发表时间：
2021
影响因子：
5
通讯作者：
Yakushi Toshiharu
中科院分区：
文献类型：
--
作者：
Miah Roni;Nina Shun;Murate Takeru;Kataoka Naoya;Matsutani Minenosuke;Matsushita Kazunobu;Yakushi Toshiharu
乙酸发酵是以乙醛为中间产物，乙醇氧化为乙酸的过程，由乙酸细菌的膜结合醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）催化。虽然ADH依赖于吡咯喹啉醌（PQQ），辅基与ALDH仍然是一个有争议的问题。研究了重氮葡萄糖酸醋杆菌PAL 5菌株ALDH对PQQ的依赖性及其在醋酸发酵中的生理作用。我们构建了PAL 5、aldFGandaldSLC两个ALDH基因簇的缺失突变株。此外，ADH的adhAB操纵子被消除，因为它显示ALDH活性。三缺失衍生物ΔaldFGHΔaldSLCΔ adhAB没有表现出ALDH活性，提示PAL 5的ALDH活性来源于这三种酶复合物。由于单基因簇缺失衍生物Δ aldFGH在乙酸发酵条件下失去了大部分ALDH活性，并且积累了比野生型高得多的乙醛，因此我们得出结论，AldFGH在PAL 5中起主要ALDH的作用。此外，PQQ生物合成基因簇（pqqABCDE）的删除消除ADH活性完全，但不影响ALDH活性。相反，异蝶呤生物合成基因缺失衍生物丧失了ALDH活性。因此，我们认为Ga. AldFGH是重氮营养葡萄糖酸醋杆菌PAL 5中主要的醛脱氢酶。·在不存在AldFGH的情况下，乙醛从乙醇中积累。·AldFGH需要钼蝶呤，而不是吡咯喹啉醌。
AbstractAcetic acid fermentation involves the oxidation of ethanol to acetic acid via acetaldehyde as the intermediate and is catalyzed by the membrane-bound alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) of acetic acid bacteria. Although ADH depends on pyrroloquinoline quinone (PQQ), the prosthetic group associated with ALDH remains a matter of debate. This study aimed to address the dependency of ALDH ofGluconacetobacter diazotrophicusstrain PAL5 on PQQ and the physiological role of ALDH in acetic acid fermentation. We constructed deletion mutant strains for both the ALDH gene clusters of PAL5,aldFGHandaldSLC. In addition, theadhABoperon for ADH was eliminated, since it shows ALDH activity. The triple-deletion derivative ΔaldFGHΔaldSLCΔadhABfailed to show ALDH activity, which suggested that ALDH activity in PAL5 is derived from these three enzyme complexes. Since the single-gene cluster deletion derivative ΔaldFGHlost most ALDH activity, and accumulated much higher acetaldehyde than wild type under acetic acid fermentation conditions, we concluded that AldFGH functions as the major ALDH in PAL5. Furthermore, deletion of the PQQ biosynthesis gene cluster (pqqABCDE) abolished ADH activity completely, but did not affect ALDH activity. Instead, the molybdopterin biosynthesis gene deletion derivatives lost ALDH activity. Thus, we concluded that the AldFGH and AldSLC complexes ofGa. diazotrophicusPAL5 require a form of molybdopterin but not PQQ for ALDH activity.Key points• AldFGH is the major aldehyde dehydrogenase in Gluconacetobacter diazotrophicus PAL5.• Acetaldehyde accumulated from ethanol in the absence of AldFGH.• Molybdopterin, rather than pyrroloquinoline quinone, is required for AldFGH.
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DOI：
10.1055/sos-sd-018-00039
发表时间：
2005
期刊：
影响因子：
--
作者：
Y. Wu
通讯作者：
Y. Wu
影响因子：
5
作者：
Kanchanarach, Watchara;Theeragool, Gunjana;Matsushita, Kazunobu
通讯作者：
Matsushita, Kazunobu
影响因子：
2.8
作者：
Marx, CJ;Lidstrom, ME
通讯作者：
Lidstrom, ME
乙酸菌中醛脱氢酶的辅基不是吡咯并喹啉醌
DOI：
10.1271/bbb.58.2082
发表时间：
1994
期刊：
Bioscience, Biotechnology, and Biochemistry
影响因子：
--
作者：
H. Takemura;T. Tsuchida;F. Yoshinaga;K. Matsushita;O. Adachi
通讯作者：
O. Adachi
氧化细菌的膜结合电子传递链连接的初级脱氢酶中存在新型辅基 PQQ
影响因子：
3.5
作者：
Tomoe Y. Nakamura;A. Jeromin;K. Mikoshiba;S. Wakabayashi
通讯作者：
S. Wakabayashi