Synergistic role of curli and cellulose in cell adherence and biofilm formation of attaching and effacing Escherichia coli and identification of Fis as a negative regulator of curli.
Synergistic role of curli and cellulose in cell adherence and biofilm formation of attaching and effacing Escherichia coli and identification of Fis as a negative regulator of curli.
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DOI：
10.1111/j.1462-2920.2008.01824.x
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发表时间：
2009-04
影响因子：
5.1
通讯作者：
Girón JA
中科院分区：
文献类型：
--
作者：
Saldaña Z;Xicohtencatl-Cortes J;Avelino F;Phillips AD;Kaper JB;Puente JL;Girón JA
卷曲是大肠杆菌和沙门氏菌的粘附菌毛。curli（csgA）和纤维素（bcsA）的表达由转录激活因子CsgD共激活。在这项研究中，我们研究了卷曲和纤维素对肠出血性（EHEC）O 157：H7和肠致病性E。大肠杆菌O 127：H6。虽然EPEC和EHEC中csgA、csgD或bcsA的单突变对细胞粘附没有显著影响，但双csgAbcsA突变体在体外对人结肠HT-29上皮细胞或牛结肠组织的粘附性显著低于单突变体或野生型菌株。在csgD突变体中过表达csgD（携带在质粒pCP 994上），而不是在单个csgA或bscA突变体中，导致EPEC和EHEC中粘附和生物膜形成的显著增加，表明卷曲和纤维素的同步过量产生增强了细菌粘附。与这一发现一致，与在组织培养基中生长相比，在培养的上皮细胞存在下csgD转录被显著激活。毒力和全球监管机构的影响，在EPEC中的curli生产的分析确定Fis（倒位刺激因子）作为一个，迄今尚未认识到的，负的转录调节csgA表达。EPEC E2348/69Δfis产生了大量的curli，而双Δ sgD突变体没有产生可检测的curli产生。我们的数据表明，curli和纤维素协同作用，有利于主机殖民，生物膜的形成，并在不同的环境中生存。
Curli are adhesive fimbriae of Escherichia coli and Salmonella enterica. Expression of curli (csgA) and cellulose (bcsA) is co-activated by the transcriptional activator CsgD. In this study, we investigated the contribution of curli and cellulose to the adhesive properties of enterohemorragic (EHEC) O157:H7 and enteropathogenic E. coli (EPEC) O127:H6. While single mutations in csgA, csgD, or bcsA in EPEC and EHEC had no dramatic effect on cell adherence, double csgAbcsA mutants were significantly less adherent than the single mutants or wild-type strains to human colonic HT-29 epithelial cells or to cow colon tissue in vitro. Over-expression of csgD (carried on plasmid pCP994) in a csgD mutant, but not in the single csgA or bscA mutants, led to significant increase in adherence and biofilm formation in EPEC and EHEC, suggesting that synchronized over-production of curli and cellulose enhances bacterial adherence. In line with this finding, csgD transcription was activated significantly in the presence of cultured epithelial cells as compared to growth in tissue culture medium. Analysis of the influence of virulence and global regulators in the production of curli in EPEC identified Fis (factor for inversion stimulation) as a, heretofore unrecognized, negative transcriptional regulator of csgA expression. An EPEC E2348/69Δfis produced abundant amounts of curli whereas a double fiscsgD mutant yielded no detectable curli production. Our data suggest that curli and cellulose act in concert to favor host colonization, biofilm formation, and survival in different environments.
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影响因子：
11.8
作者：
de Oliveira-Garcia, D;Dall'Agnol, M;Girón, JA
通讯作者：
Girón, JA
影响因子：
5.1
作者：
Gerstel, U;Römling, U
通讯作者：
Römling, U
影响因子：
3.6
作者：
Gerstel, U;Park, C;Römling, U
通讯作者：
Römling, U
影响因子：
3.2
作者：
BALL, CA;OSUNA, R;JOHNSON, RC
通讯作者：
JOHNSON, RC
影响因子：
3.2
作者：
COLLINSON, SK;EMODY, L;KAY, WW
通讯作者：
KAY, WW