Ex vivo oxidation in tissue and plasma assays of hydroxyoctadecadienoates: Z,E/E,E stereoisomer ratios.
Ex vivo oxidation in tissue and plasma assays of hydroxyoctadecadienoates: Z,E/E,E stereoisomer ratios.
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DOI：
10.1021/tx1000943
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发表时间：
2010-05-17
影响因子：
4.1
通讯作者：
Porter, Ned A.
中科院分区：
文献类型：
--
作者：
Liu, Wei;Yin, Huiyong;Akazawa, Yoko Ogawa;Yoshida, Yasukazu;Niki, Etsuo;Porter, Ned A.
生物组织和体液中亚油酸酯过氧化的主要产物是氢过氧十八碳二烯酸酯，氢过氧化物还原后，通常测定的产物是相应的羟基十八碳二烯酸酯（HODE）。HODE作为Z，E和E，E立体异构体的混合物存在于组织和体液中。通常观察到Z，E和E，E立体异构体的两个区域异构体组在18-碳链的9和13位处具有取代。已证明Z、E/E、E乘积比是评估经历过氧化的介质的还原能力的有用手段。先前报告的肝和脑等组织的HODE Z、E/E、E乘积比为0.5 - 2.0，血浆比值略高，在2.0 - 3.0之间。所报道的用于组织中HODE测定的文献方案包括均质化、在BHT存在下用硼氢化钠还原以及用KOH进行酯水解以得到游离HODE。然后进行游离酸HODE的反相HPLC或转化为TMS衍生物和GC/MS。当在方案中用三苯基膦（一种温和的还原剂）代替硼氢化钠时，HODE Z，E/E，E产物比率高得多，并且发现总HODE水平较低。使用三苯基膦作为还原剂。描述了HODE分析的修改方案（组织和血浆方法#2），其应用于组织和体液的测定。
The primary products from peroxidation of linoleate in biological tissues and fluids are the hydroperoxy octadecadienoates and the products normally assayed, after reduction of the hydroperoxides, are the corresponding hydroxy octadecadienoates (HODEs). The HODEs are found in tissues and fluids as a mixture of Z,E and E,E stereoisomers. Two regioisomeric sets of Z,E and E,E stereoisomers are normally observed with substitution at the 9 and 13 position of the 18-carbon chain. The Z,E/E,E product ratio has proved to be a useful means for assessing the reducing capacity of the medium undergoing peroxidation. The HODE Z,E/E,E product ratios previously reported for tissues such as liver and brain vary from 0.5 to 2.0 and plasma ratios are somewhat higher, between 2.0 and 3.0. The reported literature protocols for HODE assay in tissues involve homogenization, reduction with sodium borohydride in the presence of BHT, and ester hydrolysis with KOH to give the free HODEs. This is followed by either reverse-phase HPLC of the free acid HODEs or by conversion to TMS derivatives and GC/MS. When sodium borohydride is replaced in the protocol by triphenylphosphine, a gentler reducing agent, HODE Z,E/E,E product ratios are much higher and lower total HODEs levels of are found. It is proposed that inclusion of sodium borohydride in the isolation procedures leads to ex vivo reactions that are avoided if triphenylphosphine is used as the reducing agent. Modified protocols for HODE analyses (Tissue and Plasma Methods #2) are described that should be used for assays of tissues and fluids.
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影响因子：
5.9
作者：
Morrow, JD
通讯作者：
Morrow, JD
影响因子：
15
作者：
PORTER, NA;WEBER, BA;KHAN, JA
通讯作者：
KHAN, JA
影响因子：
1.8
作者：
PORTER, NA;ZURAW, PJ;SULLIVAN, JA
通讯作者：
SULLIVAN, JA
影响因子：
4.1
作者：
Culbertson, SM;Antunes, F;Porter, NA
通讯作者：
Porter, NA
DOI：
10.1016/0090-6980(92)90077-7
发表时间：
1992-08-01
期刊：
PROSTAGLANDINS
影响因子：
--
作者：
MORROW, JD;MINTON, TA;ROBERTS, LJ
通讯作者：
ROBERTS, LJ