Nuclemeter: a reaction-diffusion based method for quantifying nucleic acids undergoing enzymatic amplification.
Nuclemeter: a reaction-diffusion based method for quantifying nucleic acids undergoing enzymatic amplification.
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DOI：
10.1038/srep07335
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发表时间：
2014-12-05
影响因子：
4.6
通讯作者：
Bau HH
中科院分区：
文献类型：
--
作者：
Liu C;Sadik MM;Mauk MG;Edelstein PH;Bushman FD;Gross R;Bau HH
特定核酸序列的实时扩增和定量在医学和生物技术应用中发挥着重要作用。对于艾滋病毒等传染病，患者标本中病原体负荷的量化对于评估疾病进展和药物治疗的有效性至关重要。通常，核酸定量需要昂贵的仪器，例如实时 PCR 机，这不适合现场使用和资源匮乏的环境。本文描述了一种简单、低成本、基于反应扩散的方法，用于对酶促扩增的目标核酸进行终点定量。目标分子的数量是根据反应扩散前沿的位置推断出来的，类似于水银温度计中的温度读数。该方法经过了 HIV 病毒载量监测测试，其执行效果与传统台式方法相当。所提出的方法适用于护理点核酸定量，与多重分析和高通量处理兼容，并且无需仪器即可运行。
Real-time amplification and quantification of specific nucleic acid sequences plays a major role in medical and biotechnological applications. In the case of infectious diseases, such as HIV, quantification of the pathogen-load in patient specimens is critical to assess disease progression and effectiveness of drug therapy. Typically, nucleic acid quantification requires expensive instruments, such as real-time PCR machines, which are not appropriate for on-site use and for low-resource settings. This paper describes a simple, low-cost, reaction-diffusion based method for end-point quantification of target nucleic acids undergoing enzymatic amplification. The number of target molecules is inferred from the position of the reaction-diffusion front, analogous to reading temperature in a mercury thermometer. The method was tested for HIV viral load monitoring and performed on par with conventional benchtop methods. The proposed method is suitable for nucleic acid quantification at point of care, compatible with multiplexing and high-throughput processing, and can function instrument-free.
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影响因子：
7
作者：
Heid, CA;Stevens, J;Williams, PM
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
Christensen, DR;Hartman, LJ;Norwood, DA
通讯作者：
Norwood, DA