Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray.
Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray.
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DOI：
10.1371/journal.pone.0273106
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Tetteh, Kevin K. A.
中科院分区：
文献类型：
--
作者：
Oulton, Tate;Obiero, Joshua;Rodriguez, Isabel;Ssewanyana, Isaac;Dabbs, Rebecca A.;Bachman, Christine M.;Greenhouse, Bryan;Drakeley, Chris;Felgner, Phil L.;Stone, Will;Tetteh, Kevin K. A.
作为感染的假定血清学生物标志物的蛋白质抗原的评价已经越来越多地转向高通量、多重方法，例如蛋白质微阵列。体外转录/翻译（IVTT）系统-一种类似的高通量蛋白质表达方法-已经广泛用于蛋白质微阵列的生产，尽管来自更传统的基于全细胞的表达系统的纯化重组蛋白在生物标志物表征中也起着重要作用。在这里，我们已经进行了并排比较抗原匹配的蛋白质靶从IVTT和纯化的重组系统，在相同的蛋白质微阵列。发现对纯化的重组体的抗体应答的幅度和范围大于IVTT蛋白，并且来自不同表达系统的靶标之间的应答没有明显相关性。然而，从每个表达系统的氨基酸序列匹配的目标之间的响应更密切相关。尽管在每个表达系统中产生的抗原匹配靶之间缺乏明确的相关性，但我们的数据表明，使用任一种方法产生的蛋白质微阵列可以以依赖于上下文的方式自信地使用，尽管在比较来自对比方法的数据时应小心。
The evaluation of protein antigens as putative serologic biomarkers of infection has increasingly shifted to high-throughput, multiplex approaches such as the protein microarray. In vitro transcription/translation (IVTT) systems–a similarly high-throughput protein expression method–are already widely utilised in the production of protein microarrays, though purified recombinant proteins derived from more traditional whole cell based expression systems also play an important role in biomarker characterisation. Here we have performed a side-by-side comparison of antigen-matched protein targets from an IVTT and purified recombinant system, on the same protein microarray. The magnitude and range of antibody responses to purified recombinants was found to be greater than that of IVTT proteins, and responses between targets from different expression systems did not clearly correlate. However, responses between amino acid sequence-matched targets from each expression system were more closely correlated. Despite the lack of a clear correlation between antigen-matched targets produced in each expression system, our data indicate that protein microarrays produced using either method can be used confidently, in a context dependent manner, though care should be taken when comparing data derived from contrasting approaches.
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影响因子：
6.7
作者：
Collins, Christine R.;Withers-Martinez, Chrislaine;Hackett, Fiona;Blackman, Michael J.
通讯作者：
Blackman, Michael J.
影响因子：
4.8
作者：
Dopp, Jared L.;Jo, Yeong Ran;Reuel, Nigel F.
通讯作者：
Reuel, Nigel F.
影响因子：
3.1
作者：
Adu B;Issahaque QA;Sarkodie-Addo T;Kumordjie S;Kyei-Baafour E;Sinclear CK;Eyia-Ampah S;Owusu-Yeboa E;Theisen M;Dodoo D
通讯作者：
Dodoo D
DOI：
10.1073/pnas.0408782102
发表时间：
2005-01-18
影响因子：
11.1
作者：
Davies, DH;Liang, XW;Felgner, PL
通讯作者：
Felgner, PL
DOI：
10.1073/pnas.0408725102
发表时间：
2005-04-05
影响因子：
11.1
作者：
Drakeley, CJ;Corran, PH;Riley, EM
通讯作者：
Riley, EM