Temperature, light and nitrate sensing coordinate Arabidopsis seed dormancy cycling, resulting in winter and summer annual phenotypes.
Temperature, light and nitrate sensing coordinate Arabidopsis seed dormancy cycling, resulting in winter and summer annual phenotypes.
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DOI：
10.1111/tpj.12186
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发表时间：
2013-06
期刊：
影响因子：
--
通讯作者：
Finch-Savage WE
中科院分区：
文献类型：
--
作者：
Footitt S;Huang Z;Clay HA;Mead A;Finch-Savage WE
种子利用环境线索来感知季节和周围环境，从而启动植物的生命周期。这个过程背后的休眠循环被广泛描述，但分子机制在很大程度上是未知的。为了解决这一问题，我们选择了一系列的代表性基因，从实验室发表的阵列实验，并研究了它们的表达模式在种子中的拟南芥生态型与对比的生命周期在每年的休眠周期在外地。我们展示了在实验室中确定的机制是如何协调的土壤环境，以确定导致冬季和夏季年度表型的休眠周期。我们的研究结果与种子对季节性温度模式的特异性反应一致（时间感测），涉及指示正确季节的基因DELAY OF GEMINATION 1（DOG 1），以及同时的时间驱动的增选机制，其通过硝酸盐转运蛋白1的CBL相互作用蛋白激酶23（CIPK 23）磷酸化来感测空间信号，即硝酸盐（NRT1.1）和光，通过光敏色素A（PHYA）。在这两个生态型研究，当所有三个基因有低表达有增强赤霉素3 β-羟化酶1（GA 3 ox 1）的表达，挖掘的种子有可能在实验室发芽，并开始出苗后发生土壤扰动（暴露于光）在现场。与DOG 1不同，开花时间的母亲（MFT）的表达在两种生态型的种子中具有相反的热反应，表明在决定其不同的休眠循环表型中的作用。
Seeds use environmental cues to sense the seasons and their surroundings to initiate the life cycle of the plant. The dormancy cycling underlying this process is extensively described, but the molecular mechanism is largely unknown. To address this we selected a range of representative genes from published array experiments in the laboratory, and investigated their expression patterns in seeds of Arabidopsis ecotypes with contrasting life cycles over an annual dormancy cycle in the field. We show how mechanisms identified in the laboratory are coordinated in response to the soil environment to determine the dormancy cycles that result in winter and summer annual phenotypes. Our results are consistent with a seed-specific response to seasonal temperature patterns (temporal sensing) involving the gene DELAY OF GERMINATION 1 (DOG1) that indicates the correct season, and concurrent temporally driven co-opted mechanisms that sense spatial signals, i.e. nitrate, via CBL-INTERACTING PROTEIN KINASE 23 (CIPK23) phosphorylation of the NITRATE TRANSPORTER 1 (NRT1.1), and light, via PHYTOCHROME A (PHYA). In both ecotypes studied, when all three genes have low expression there is enhanced GIBBERELLIN 3 BETA-HYDROXYLASE 1 (GA3ox1) expression, exhumed seeds have the potential to germinate in the laboratory, and the initiation of seedling emergence occurs following soil disturbance (exposure to light) in the field. Unlike DOG1, the expression of MOTHER of FLOWERING TIME (MFT) has an opposite thermal response in seeds of the two ecotypes, indicating a role in determining their different dormancy cycling phenotypes.
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影响因子：
2.1
作者：
Botto, JF;Sánchez, RA;Casal, JJ
通讯作者：
Casal, JJ
影响因子：
9.4
作者：
Donohue, Kathleen;Heschel, M. Shane;Chiang, George C. K.
通讯作者：
Chiang, George C. K.
影响因子：
7.3
作者：
DERKX, MPM;KARSSEN, CM
通讯作者：
KARSSEN, CM
影响因子：
7.2
作者：
Finch-Savage, William E.;Cadman, Cassandra S. C.;Hilhorst, Henk W. M.
通讯作者：
Hilhorst, Henk W. M.
DOI：
10.1139/b72-049
发表时间：
1972-01-01
期刊：
CANADIAN JOURNAL OF BOTANY
影响因子：
--
作者：
BASKIN, JM;BASKIN, CC
通讯作者：
BASKIN, CC