Distribution of fibronectin and laminin in basal cell epitheliomas.
Distribution of fibronectin and laminin in basal cell epitheliomas.
复制标题
基底细胞上皮瘤中纤连蛋白和层粘连蛋白的分布。
DOI：
10.1111/1523-1747.ep12555545
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
G. Balian
中科院分区：
文献类型：
--
作者：
D. Nelson;C. Little;G. Balian
用间接免疫荧光法检测基底细胞上皮瘤中纤维连接蛋白（FN）和层粘连蛋白（LM）的分布。FN是一种促进细胞与细胞外基质相互作用的糖蛋白，存在于真皮-表皮交界处（DEJ）和整个真皮中，但在正常表皮中不存在。LM是一种非胶原性基底膜蛋白，在上皮与IV型胶原的粘附中发挥作用，通常存在于DEJ中，但在表皮中不存在。FN和LM在上皮分化中的作用尚未确定。因此，FN和LM在上皮来源的肿瘤中的分布通过间接免疫荧光进行了研究。使用亲和纯化的抗体FN和LM，和适当的荧光素标记的第二抗体，正常皮肤和7基底细胞肿瘤进行了检查。通过免疫荧光，恶性基底细胞巢被线性LM染色包围。FN免疫荧光在所有肿瘤的结缔组织间质中强烈。五个肿瘤也显示FN染色的肿瘤细胞巢内，其中4个也LM阳性的肿瘤块。这些免疫荧光结果表明，表皮瘤形成可能与FN和LM分布的改变有关。
The distribution of fibronectin (FN) and laminin (LM) in basal cell epithelioma was evaluated by indirect immunofluorescence. FN is a glycoprotein which promotes interaction between cells and the extracellular matrix, and is present at the dermal-epidermal junction (DEJ) and throughout the dermis, but absent in the normal epidermis. LM, a noncollagenous basement membrane protein, plays a role in epithelial adhesion to type IV collagen, and is normally present in the DEJ, but absent in the epidermis. The role of FN and LM in epithelial differentiation has not been established. Therefore, the distribution of FN and LM in a tumor of epithelial origin was studied by indirect immunofluorescence. Using affinity-purified antibodies to FN and LM, and the appropriate fluorescein-conjugated second antibodies, normal skin and 7 basal cell tumors were examined. By immunofluorescence, nests of malignant basal cells were surrounded by linear LM staining. FN immunofluorescence was intense throughout the connective tissue stroma of all tumors. Five tumors also showed FN staining within the nests of neoplastic cells, and 4 of these were also LM-positive in the tumor bulk. These immunofluorescent findings suggest that epidermal neoplasia can be associated with alterations in the distribution of FN and LM.
影响因子：
6.5
作者：
FLEISCHMAJER, R;PERLISH, JS;TIMPL, R
通讯作者：
TIMPL, R