The open reading frame S of visna virus genome is a trans-activating gene.
The open reading frame S of visna virus genome is a trans-activating gene.
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维斯纳病毒基因组的开放阅读框S是反式激活基因。
DOI：
10.1016/0042-6822(89)90524-2
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发表时间：
1989
期刊：
影响因子：
3.7
通讯作者：
R. Vigne
中科院分区：
文献类型：
--
作者：
I. Gourdou;V. Mazarin;G. Quérat;N. Sauze;R. Vigne
在感染绵羊细胞后不久，Visna病毒的表达首先表现为1.2和1.6kb的两个多剪接转录本的积累，目前我们根据它们的确切长度将它们重新命名为1.4和1.7kb。早期1.4-kb的mRNA编码一种蛋白质，它增加了Visna病毒长末端重复序列(反式激活)指示的转录本的水平。Thistrans激活蛋白以前被称为VEP1，目前根据该蛋白与猴免疫缺陷病毒和人类免疫缺陷病毒2型的重组基因产物的氨基酸序列显著同源性而更名为该基因的产物。本研究克隆了1.7kb的mRNA，并进行了测序和体外翻译。它全长1,491个核苷酸，包含两个短的开放阅读框(ORF)、TAT(以前的ORF S)和REVE，它是早期1.4kb信使核糖核酸的两部分特异作用基因。Visna病毒的atA基因编码一种11 kDa的蛋白，在瞬时表达试验中，该蛋白与rev基因产物一样，对转录具有积极的反式作用。
Soon after infection of ovine cell cultures, visna virus expression is first indicated by the accumulation of two multispliced transcripts of 1.2 and 1.6 kb that at present we have renamed 1.4 and 1.7 kb according to their exact length. The early 1.4-kb mRNA encodes for a protein which increases the level of transcripts directed from visna virus long terminal repeat (trans-activation). Thistrans-activating protein was previously called VEP1 and at present is renamed as the product of therevgene according to significant amino acid sequence homologies between this protein and therevgene products of simian immunodeficiency virus and human immunodeficiency virus type 2. In this study, the 1.7kb mRNA was cloned, sequenced, andin vitrotranslated. It is 1491 nucleotides long, contains two short open reading frames, (orfs), tat (previously orf S) andrevwhich is the bipartitetrans-acting gene specific for the early 1.4-kb mRNA. Thetatgene of visna virus encodes for a protein of 11 kDa which in transient expression assays has a positivetrans-acting effect on transcription as the rev gene product does.
维斯纳病毒的顺式和反式转录调控。
DOI：
10.1126/science.2990051
发表时间：
1985
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hess,JL;Clements,JE;Narayan,O
通讯作者：
Narayan,O