Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus
Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus
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脉冲场凝胶电泳基因组 DNA 指纹作为流行病学标志物研究耐甲氧西林金黄色葡萄球菌引起的医院感染
DOI：
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发表时间：
1991
影响因子：
9.4
通讯作者：
'. Juntakeuchi
中科院分区：
文献类型：
--
作者：
S. Ichiyama;M. Ohta;K. Shimokata;N. Kato;'. Juntakeuchi
在这项研究中，我们利用脉冲场凝胶电泳（PFGE）比较了耐甲氧西林金黄色葡萄球菌（MRSA）分离株的基因组DNA指纹图谱。smi酶切的染色体片段最适合PFGE分离。smi将基因组DNA切割成15到20个片段，大小从30到1500 kb不等。在日本6家不同医院的111株感染和定植MRSA分离株中鉴定出31种不同的片段模式。在PFGE基因组分型的基础上，我们对名古屋大学医院住院患者中爆发的院内MRSA感染进行了流行病学调查。从18名感染患者和1名定植医院人员中分离的20株菌株中鉴定出10种染色体消化。根据限制模式，我们发现这些菌株在13例患者中引起了流行感染。两种类型（1型和4型）的菌株涉及5名患者的死亡。其他感染是零星的。染色体消化模式的清晰度和多态性使我们能够区分无法通过抗生素谱或质粒分析区分的分离株。因此，通过PFGE对基因组DNA指纹图谱进行分类被认为是调查院内MRSA感染的来源、传播和传播的一种有用方法。
In this study, we have compared genomic DNA fingerprintings among isolates of methicillin-resistant Staphylococcus aureus (MRSA) by using pulsed-field gel electrophoresis (PFGE). Chromosomal fragments digested with SmaI were most suitable for the PFGE separation. SmaI cut genomic DNA into 15 to 20 fragments whose sizes ranged from about 30 to 1,500 kb. Thirty-one distinctive fragment patterns were identified in 111 infecting and colonizing MRSA isolates from six different hospitals in Japan. On the basis of the genomic typing by PFGE, we performed an epidemiological investigation of an outbreak of nosocomial MRSA infections among inpatients in Nagoya University Hospital. Ten types of chromosomal digestion were identified in the 20 strains isolated from 18 infected patients and 1 from colonized hospital personnel. According to the restriction patterns, we found that four types of these strains had caused epidemic infections among 13 patients in the outbreak. Two types (types 1 and 4) of the strains were involved in the death of five patients. The other infections were sporadic. The clarity and polymorphism of the chromosomal digestion patterns enabled us to discriminate between isolates which could not be differentiated by antibiogram or plasmid analysis. Classification of the genomic DNA fingerprinting patterns by PFGE is therefore proposed as a useful method for investigating the source, transmission, and spread of nosocomial MRSA infections.
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多重耐药金黄色葡萄球菌：医院感染的引入、传播和演变。
影响因子：
39.2
作者：
Locksley,RM;Cohen,ML;Quinn,TC;Tompkins,LS;Coyle,MB;Kirihara,JM;Counts,GW
通讯作者：
Counts,GW
大肠杆菌 K12 基因组的物理图谱。
DOI：
10.1126/science.3296194
发表时间：
1987
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Smith,CL;Econome,JG;Schutt,A;Klco,S;Cantor,CR
通讯作者：
Cantor,CR
大 DNA 分子的纯化、特异性片段化和分离。
影响因子：
--
作者：
Smith,CL;Cantor,CR
通讯作者：
Cantor,CR
大肠杆菌生物操纵子的物理图。
影响因子：
3.5
作者：
Szybalski,EH;Szybalski,W
通讯作者：
Szybalski,W