Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria.
Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria.
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DOI：
10.1038/s41467-022-29007-y
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发表时间：
2022-03-21
影响因子：
16.6
通讯作者：
Sockett RE
中科院分区：
文献类型：
--
作者：
Banks EJ;Valdivia-Delgado M;Biboy J;Wilson A;Cadby IT;Vollmer W;Lambert C;Lovering AL;Sockett RE
肽聚糖水解酶有助于弯曲杆菌属和螺杆菌属细菌中螺旋细胞形状的产生，而细胞骨架或周壁蛋白决定柄杆菌属和弧菌属的弯曲的弧菌样细胞形状。在这里，我们确定了一种肽聚糖水解酶的弧菌形的捕食性细菌噬菌蛭弧菌的侵入和复制内的革兰氏阴性猎物细菌的周质。蛋白质Bd 1075在B中产生细胞曲率。在球形猎物内部生长时，通过在捕食者细胞壁上施加LD-羧肽酶活性，对食菌性细菌产生影响。Bd 1075定位于B的外凸面。这种不对称定位需要在蛋白质C-末端的核转运因子2样（NTF2）结构域。我们解决了晶体结构的Bd1075，这是单体与其他LD-羧肽酶的关键区别。杆状Δ bd1075突变体入侵猎物比弯曲的野生型捕食者更慢，并从内部拉伸入侵猎物。因此，我们认为B. bacteriovorus有助于捕食适应性。噬菌蛭弧菌（Bdellovibrio bacteriovorus）是一种捕食性细菌，它侵入其他细菌的周质内并在其中复制，其细胞具有特征性的弯曲杆状。在这里，班克斯等人表明，肽聚糖水解酶是弯曲形状所必需的，这有利于猎物细胞的入侵。
Peptidoglycan hydrolases contribute to the generation of helical cell shape in Campylobacter and Helicobacter bacteria, while cytoskeletal or periskeletal proteins determine the curved, vibrioid cell shape of Caulobacter and Vibrio. Here, we identify a peptidoglycan hydrolase in the vibrioid-shaped predatory bacterium Bdellovibrio bacteriovorus which invades and replicates within the periplasm of Gram-negative prey bacteria. The protein, Bd1075, generates cell curvature in B. bacteriovorus by exerting LD-carboxypeptidase activity upon the predator cell wall as it grows inside spherical prey. Bd1075 localizes to the outer convex face of B. bacteriovorus; this asymmetric localization requires a nuclear transport factor 2-like (NTF2) domain at the protein C-terminus. We solve the crystal structure of Bd1075, which is monomeric with key differences to other LD-carboxypeptidases. Rod-shaped Δbd1075 mutants invade prey more slowly than curved wild-type predators and stretch invaded prey from within. We therefore propose that the vibrioid shape of B. bacteriovorus contributes to predatory fitness. Cells of the predatory bacterium Bdellovibrio bacteriovorus, which invades and replicates within the periplasm of other bacteria, have a characteristic curved rod shape. Here, Banks et al. show that a peptidoglycan hydrolase is required for the curved shape, and this facilitates invasion of prey cells.
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影响因子：
6.7
作者：
Frirdich E;Biboy J;Adams C;Lee J;Ellermeier J;Gielda LD;Dirita VJ;Girardin SE;Vollmer W;Gaynor EC
通讯作者：
Gaynor EC
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
DOI：
10.1073/pnas.1317497111
发表时间：
2014-04-22
影响因子：
11.1
作者：
Amir, Ariel;Babaeipour, Farinaz;Jun, Suckjoon
通讯作者：
Jun, Suckjoon
影响因子：
64.5
作者：
Bartlett TM;Bratton BP;Duvshani A;Miguel A;Sheng Y;Martin NR;Nguyen JP;Persat A;Desmarais SM;VanNieuwenhze MS;Huang KC;Zhu J;Shaevitz JW;Gitai Z
通讯作者：
Gitai Z