Spatial Correlations and Distribution of Competence Gene Expression in Biofilms of Streptococcus mutans.
Spatial Correlations and Distribution of Competence Gene Expression in Biofilms of Streptococcus mutans.
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DOI：
10.3389/fmicb.2020.627992
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发表时间：
2020
影响因子：
5.2
通讯作者：
Hagen SJ
中科院分区：
文献类型：
--
作者：
Ishkov IP;Kaspar JR;Hagen SJ
变形链球菌是人类口腔生物膜中的一种重要病原体。它表达的毒性行为与其由comX控制的遗传能力调控有关。comX的表达受两种扩散信号肽（CSP和XIP）以及其他环境因素（如pH和氧化应激）的调节。变形链球菌能力对环境输入的敏感性可能在微观长度尺度上发生变化，这引发了一个问题，即生物膜环境是否创造了微生态环境，在微生态环境中，能力和相关表型集中，导致变形链球菌毒力行为的空间聚类。我们使用双光子显微镜来表征生物膜中单个变形链球菌细胞中comX表达的空间分布。通过分析comX活动的相关性，我们测试了可能表明局部能力微环境的空间聚类。我们的数据表明，这两种能力信号肽都能有效地通过生物膜扩散。XIP引发了全民响应。CSP从均匀分布的细胞亚群中触发类似泊松的、空间随机的comX反应。我们的数据表明，能力微环境（如果存在的话）足够小，单个细胞的表型不会聚集在一起，也不会比浮游生物培养中发生的更大程度上相关。
Streptococcus mutans is an important pathogen in the human oral biofilm. It expresses virulent behaviors that are linked to its genetic competence regulon, which is controlled by comX. Expression of comX is modulated by two diffusible signaling peptides, denoted CSP and XIP, and by other environmental cues such as pH and oxidative stress. The sensitivity of S. mutans competence to environmental inputs that may vary on microscopic length scales raises the question of whether the biofilm environment creates microniches where competence and related phenotypes are concentrated, leading to spatial clustering of S. mutans virulence behaviors. We have used two-photon microscopy to characterize the spatial distribution of comX expression among individual S. mutans cells in biofilms. By analyzing correlations in comX activity, we test for spatial clustering that may suggest localized competence microenvironments. Our data indicate that both competence-signaling peptides diffuse efficiently through the biofilm. XIP elicits a population-wide response. CSP triggers a Poisson-like, spatially random comX response from a subpopulation of cells that is homogeneously dispersed. Our data indicate that competence microenvironments if they exist are small enough that the phenotypes of individual cells are not clustered or correlated to any greater extent than occurs in planktonic cultures.
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影响因子：
2.1
作者：
Aspiras, MB;Ellen, RP;Cvitkovitch, DG
通讯作者：
Cvitkovitch, DG
影响因子：
2
作者：
Hagen SJ;Son M
通讯作者：
Son M
影响因子：
4.5
作者：
Islam, Md. Maidul;Chakraborty, Maharudra;Mukhopadhyay, Subrata
通讯作者：
Mukhopadhyay, Subrata
影响因子：
3.4
作者：
Kiskowski, Maria A.;Hancock, John F.;Kenworthy, Anne K.
通讯作者：
Kenworthy, Anne K.
DOI：
10.1038/s41396-020-00789-7
发表时间：
2021-03
期刊：
The ISME journal
影响因子：
--
作者：
Kaspar JR;Lee K;Richard B;Walker AR;Burne RA
通讯作者：
Burne RA