Epidermal development in Xenopus laevis: the definition of a monoclonal antibody to an epidermal marker.
Epidermal development in Xenopus laevis: the definition of a monoclonal antibody to an epidermal marker.
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非洲爪蟾的表皮发育：针对表皮标记物的单克隆抗体的定义。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
E. Jones
中科院分区：
文献类型：
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作者：
E. Jones
描述了一种单克隆抗体2F7.C7，其识别无尾两栖类和有尾目两栖类共有的表皮抗原。该抗原具有高分子量，并且在1/2 1/2阶段首先在表皮细胞内部然后在表皮细胞表面上表达。在假定的神经组织上不表达。它在幼虫表皮的所有细胞上表达，包括纤毛和非纤毛细胞以及吸盘。通过花生凝集素封闭实验研究了表皮抗原与“表粘蛋白”的关系。这些研究表明2F7.C7识别的分子不是表粘蛋白，或者其表位远离凝集素结合位点。研究使用这种抗体在移植和三明治实验进行了讨论，并提出了在爪蟾表皮发育的总体方案。
A monoclonal antibody 2F7.C7 which identifies an epidermal antigen common to both anuran and urodele amphibia is described. This antigen has a high molecular weight and is expressed at stage 12 1/2 first inside and then on the surface of epidermal cells. It is not expressed on presumptive nervous tissue. It is expressed on all cells of the larval epidermis including ciliated and nonciliated cells and sucker. The relationship of the epidermal antigen to 'epimucin' is investigated by blocking experiments using Peanut agglutinin. These studies suggest that either the molecule recognized by 2F7.C7 is not epimucin, or that its epitope is far away from the lectin-binding site. Studies using this antibody in grafting and sandwich experiments are discussed and an overall scheme of epidermal development in Xenopus is presented.
影响因子：
2.9
作者：
G. Tuszynski;L. Knight;E. Kornecki;S. Srivastava
通讯作者：
G. Tuszynski;L. Knight;E. Kornecki;S. Srivastava