Role of mutational reversions and fitness restoration in Zika virus spread to the Americas.
Role of mutational reversions and fitness restoration in Zika virus spread to the Americas.
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DOI：
10.1038/s41467-020-20747-3
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发表时间：
2021-01-26
影响因子：
16.6
通讯作者：
Weaver SC
中科院分区：
文献类型：
--
作者：
Liu J;Liu Y;Shan C;Nunes BTD;Yun R;Haller SL;Rafael GH;Azar SR;Andersen CR;Plante K;Vasilakis N;Shi PY;Weaver SC
Zika virus (ZIKV) emerged from obscurity in 2013 to spread from Asia to the South Pacific and the Americas, where millions of people were infected, accompanied by severe disease including microcephaly following congenital infections. Phylogenetic studies have shown that ZIKV evolved in Africa and later spread to Asia, and that the Asian lineage is responsible for the recent epidemics in the South Pacific and Americas. However, the reasons for the sudden emergence of ZIKV remain enigmatic. Here we report evolutionary analyses that revealed four mutations, which occurred just before ZIKV introduction to the Americas, represent direct reversions of previous mutations that accompanied earlier spread from Africa to Asia and early circulation there. Our experimental infections of Aedes aegypti mosquitoes, human cells, and mice using ZIKV strains with and without these mutations demonstrate that the original mutations reduced fitness for urban, human-amplifed transmission, while the reversions restored fitness, increasing epidemic risk. These findings include characterization of three transmission-adaptive ZIKV mutations, and demonstration that these and one identified previously restored fitness for epidemic transmission soon before introduction into the Americas. The initial mutations may have followed founder effects and/or drift when the virus was introduced decades ago into Asia. The trajectory of the emergence of Zika virus (ZIKV) into the Americas remains unclear. Here, the authors find that four mutations originated before ZIKV introduction to the Americas are direct reversions of previous mutations that accompanied spread many decades ago from ZIKV’s native Africa to Asia, and show in experimental infections of mosquitoes, human cells, and mice that the original mutations reduced fitness for urban transmission, while the reversions restored fitness, likely increasing epidemic risk.
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DOI：
10.1056/nejmoa1602412
发表时间：
2016-12-15
期刊：
The New England journal of medicine
影响因子：
--
作者：
Brasil P;Pereira JP Jr;Moreira ME;Ribeiro Nogueira RM;Damasceno L;Wakimoto M;Rabello RS;Valderramos SG;Halai UA;Salles TS;Zin AA;Horovitz D;Daltro P;Boechat M;Raja Gabaglia C;Carvalho de Sequeira P;Pilotto JH;Medialdea-Carrera R;Cotrim da Cunha D;Abreu de Carvalho LM;Pone M;Machado Siqueira A;Calvet GA;Rodrigues Baião AE;Neves ES;Nassar de Carvalho PR;Hasue RH;Marschik PB;Einspieler C;Janzen C;Cherry JD;Bispo de Filippis AM;Nielsen-Saines K
通讯作者：
Nielsen-Saines K
DOI：
10.4269/ajtmh.17-0263
发表时间：
2017-11
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Duggal NK;Ritter JM;McDonald EM;Romo H;Guirakhoo F;Davis BS;Chang GJ;Brault AC
通讯作者：
Brault AC
影响因子：
56.9
作者：
Aubry, Fabien;Dabo, Stephanie;Lambrechts, Louis
通讯作者：
Lambrechts, Louis
DOI：
10.2807/1560-7917.es.2016.21.38.30347
发表时间：
2016-09-22
期刊：
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
影响因子：
--
作者：
Maurer-Stroh S;Mak TM;Ng YK;Phuah SP;Huber RG;Marzinek JK;Holdbrook DA;Lee RT;Cui L;Lin RT
通讯作者：
Lin RT
影响因子：
56.3
作者：
Hill, Sarah C.;Vasconcelos, Jocelyne;Faria, Nuno R.
通讯作者：
Faria, Nuno R.