Technological development of intracellular polysilicon–chromium–gold chips for orthogonal chemical functionalization
Technological development of intracellular polysilicon–chromium–gold chips for orthogonal chemical functionalization
复制标题
正交化学功能化细胞内多晶硅-铬-金芯片技术开发
DOI：
10.1016/j.snb.2014.11.077
复制
发表时间：
2015
影响因子：
8.4
通讯作者：
J. Plaza
中科院分区：
文献类型：
--
作者：
Sara Durán;M. Duch;Tania Patiño;Á. Torres;O. Penon;R. Gómez;L. Barrios;J. Esteve;C. Nogués;L. Pérez;J. Plaza
微电子技术的进步越来越集中于获得用于纳米医学和细胞生物学等领域的新芯片，利用微电子技术制造具有细胞尺寸和多种特征的设备的能力。在这里，我们报告了一种多材料芯片的制造技术，使用多晶硅和金作为器件层，用作双功能电池内部化器件。在我们的情况下，主要的技术挑战之一是克服这两种材料之间的低粘附性，特别是因为它们的接触面积很小，仅为9μm2。因此，为了绕过这一困难，在两者之间沉积了一层铬粘附层。在制造后，遵循这种设计的装置可以成功地内化到活的巨噬细胞中，而不会影响它们的生存能力。在一个设备中拥有多个材料层的优势是有可能呈现多任务芯片，因为一旦它们被适当地功能化，我们就可以为芯片提供多功能的能力。因此，作为概念的验证，利用正交化学方法通过自组装单分子膜将小麦胚芽凝集素(WGA)和刀豆蛋白(ConA)两种不同的蛋白质固定在芯片表面。这项工作的结果表明，胞内多晶硅-铬-金芯片的制备工艺可控，具有双功能，并且没有细胞毒性。最后，两种不同的染料(Oregon Green®488和BODIPY®581/591)被用来对多材料芯片的每个表面进行双功能化，以证明功能芯片也可以内化在活细胞中。这些设备作为生物传感、药物输送和诊断的细胞内功能平台具有广阔的前景。
Increasingly, advances in microtechnologies are focused on obtaining new chips intended for applications in fields such as nanomedicine and cell biology, taking advantage of the ability of microelectronics to manufacture devices with cell dimensions and a large variety of features. Here, we report a technology for the fabrication of multi-material chips, using polysilicon and gold as device layers, to be used as bi-functional cell-internalizable devices. In our case, one of the main technological challenges is to overcome the low adherence between these two materials, especially because of their small contact-area, only 9 μm2. Thus, in order to circumvent this difficulty a chromium adherent-layer was deposited in between. After fabrication, the devices following this design can be successfully internalized inside living macrophages without affecting their viability. The advantage of having multiple material layers in one device is the potential to render multi-tasking chips, as once they are appropriately functionalized, we can provide the chip the ability of being multi-functional. Hence, and as a proof of concept, two different proteins, Wheat Germ Agglutinin (WGA) and Concanavalin (ConA), were immobilized on the chip surface through self-assembled monolayers using orthogonal chemistry. The results of this work show a well-controlled fabrication, the bi-functional capabilities and no cell-toxicity of intracellular polysilicon–chromium–gold chips. Eventually, two different dyes (Oregon Green®488 and BODIPY®581/591) were used to bi-functionalize each surface of the multi-material chip in order to demonstrate that functional chips can also be internalized in living cells. These devices have a promising future as intracellular functional platforms for biosensing, drug delivery and diagnosis.
DOI：
10.1021/la3010079
发表时间：
2012-07-03
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Tang JL;Schoenwald K;Potter D;White D;Sulchek T
通讯作者：
Sulchek T
DOI：
10.1002/wnan.82
发表时间：
2011-01
影响因子：
8.6
作者：
Hu, Ye;Fine, Daniel H.;Tasciotti, Ennio;Bouamrani, Ali;Ferrari, Mauro
通讯作者：
Ferrari, Mauro
DOI：
10.1039/c3tb00048f
发表时间：
2013
期刊：
Journal of materials chemistry. B
影响因子：
--
作者：
Kam KR;Desai TA
通讯作者：
Desai TA