The virion N protein of infectious bronchitis virus is more phosphorylated than the N protein from infected cell lysates.
The virion N protein of infectious bronchitis virus is more phosphorylated than the N protein from infected cell lysates.
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DOI：
10.1016/j.virol.2005.04.029
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发表时间：
2005-08-15
期刊：
影响因子：
3.7
通讯作者：
Collisson EW
中科院分区：
文献类型：
--
作者：
Jayaram J;Youn S;Collisson EW
由于传染性支气管炎病毒（IBV）核衣壳蛋白（N）的磷酸化可以调节其在病毒复制中的多种作用，因此检测了N磷酸化的动力学。32 P-正磷酸标记和Western印迹分析证实，N是唯一被磷酸化的病毒蛋白。用32 P-正磷酸脉冲标记表明，IBV N蛋白在病毒粒子中被磷酸化，以及在感染鸡胚肾细胞或Vero细胞期间的所有时间。用兔抗IBV N多克隆抗体免疫沉淀IBV N蛋白后进行的脉冲追踪分析表明，N蛋白上的磷酸盐至少能稳定1 h。与鸡胚肾细胞的细胞裂解物中N的32 P：3 H cpm比相比，用32 P-正磷酸盐和3 H-亮氨酸同时标记，发现病毒体中N的32 P：3 H计数/分钟（cpm）比增加了3.5倍，而在Vero细胞中，病毒体中N的32 P：3 H cpm比是细胞裂解物中N的32 P：3 H cpm比的10.5倍。这些研究与IBV N的磷酸化在病毒颗粒的组装或成熟中起作用一致。
Because phosphorylation of the infectious bronchitis virus (IBV) nucleocapsid protein (N) may regulate its multiple roles in viral replication, the dynamics of N phosphorylation were examined. 32P-orthophosphate labeling and Western blot analyses confirmed that N was the only viral protein that was phosphorylated. Pulse labeling with 32P-orthophosphate indicated that the IBV N protein was phosphorylated in the virion, as well as at all times during infection in either chicken embryo kidney cells or Vero cells. Pulse-chase analyses followed by immunoprecipitation of IBV N proteins using rabbit anti-IBV N polyclonal antibody demonstrated that the phosphate on the N protein was stable for at least 1 h. Simultaneous labeling with 32P-orthophosphate and 3H-leucine identified a 3.5-fold increase in the 32P:3H counts per minute (cpm) ratio of N in the virion as compared to the 32P:3H cpm ratio of N in the cell lysates from chicken embryo kidney cells, whereas in Vero cells the 32P:3H cpm ratio of N from the virion was 10.5-fold greater than the 32P:3H cpm ratio of N from the cell lysates. These studies are consistent with the phosphorylation of the IBV N playing a role in assembly or maturation of the viral particle.
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影响因子：
5.4
作者：
Nguyen, VP;Hogue, BG
通讯作者：
Hogue, BG
影响因子：
5.4
作者：
Narayanan, K;Maeda, A;Makino, S
通讯作者：
Makino, S
影响因子：
2.7
作者：
BINGHAM, RW
通讯作者：
BINGHAM, RW
影响因子：
5
作者：
PERLMAN, S;RIES, D;STOLTZFUS, CM
通讯作者：
STOLTZFUS, CM
DOI：
10.1093/protein/13.6.437
发表时间：
2000-06-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
Raha, T;Samal, E;Chattopadhyay, DJ
通讯作者：
Chattopadhyay, DJ