Systemic endotoxin levels in chronic indolent periodontal infections.
Systemic endotoxin levels in chronic indolent periodontal infections.
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DOI：
10.1111/j.1600-0765.2008.01169.x
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发表时间：
2010-02
影响因子：
3.5
通讯作者：
Novak MJ
中科院分区：
文献类型：
--
作者：
Ebersole JL;Stevens J;Steffen MJ;Dawson Iii D;Novak MJ
牙周病与各种全身性疾病的风险增加有关。对这种联系的一个合理的生物学解释包括口腔病原体有机会易位到循环中，这是口腔上皮完整性破坏的结果。本研究改进了一种用于检测无痛性牙周感染受试者血清中内毒素活性的方法。QCL®动态显色试验（Cambrex）是内毒素活性的动态测量。来自211名参加产科和牙周病治疗试验的牙周炎孕妇的血清被用于进一步开发该检测方法，并评估可能伴随慢性牙周炎低水平菌血症的内毒素活性的检测。我们优化了系统，以提高检测的灵敏度和重现性。改进的系统能够在> 0.0125 EU/mL下检测血清中的内毒素活性。在基线（妊娠13-16周），35.5%的女性内毒素活性呈阳性（1.62 ± 2.21;范围：0.38-15 EU/mL）。本报告描述了血清中内毒素活性的灵敏测量。该程序使我们能够记录这种微生物毒力因子在慢性感染（如牙周病）患者血清中的水平。
Periodontal disease has been linked with an increased risk of various systemic diseases. A plausible biologic explanation for this link includes the opportunity for oral pathogens to translocate to the circulation as a result of breakdown in integrity of the oral epithelium. This study refined a methodology used to detect endotoxin activity in the serum of subjects with indolent periodontal infections. The QCL® Kinetic Chromogenic Assay (Cambrex) is a kinetic measure of endotoxin activity. Sera from 211 pregnant women with periodontitis enrolled in the Obstetrics and Periodontal Therapy Trial were used to develop the assay further and to evaluate the detection of endotoxin activity that might accompany a low-level bacteremia in chronic periodontitis. We optimized the system to increase the sensitivity and reproducibility of the assay. The refined system was able to detect endotoxin activity in serum at > 0.0125 EU/mL. At baseline (13–16 wk of gestation), 35.5% of the women were positive for endotoxin activity (1.62 ± 2.21; range: 0.38–15 EU/mL). This report describes a sensitive measure of endotoxin activity in serum. The procedure allowed us to document levels of this microbial virulence factor in serum of individuals with indolent infections such as periodontal disease.
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DOI：
10.2174/1874210600802010049
发表时间：
2008
期刊：
The open dentistry journal
影响因子：
--
作者：
Kirakodu SS;Govindaswami M;Novak MJ;Ebersole JL;Novak KF
通讯作者：
Novak KF
影响因子：
4.3
作者：
da Silva, RM;Caugant, DA;Olsen, I
通讯作者：
Olsen, I
影响因子：
37.8
作者：
Desvarieux, M;Demmer, RT;Papapanou, PN
通讯作者：
Papapanou, PN
DOI：
10.1016/j.lab.2005.05.006
发表时间：
2005-09-01
期刊：
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
影响因子：
--
作者：
Aue, G;Carroll, N;Horne, MK
通讯作者：
Horne, MK
DOI：
10.1067/mlc.2002.127170
发表时间：
2002-10-01
期刊：
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
影响因子：
--
作者：
Jellema, WT;Veerman, DP;Van Lieshout, JJ
通讯作者：
Van Lieshout, JJ