BnHO1, a haem oxygenase-1 gene from Brassica napus, is required for salinity and osmotic stress-induced lateral root formation.
BnHO1, a haem oxygenase-1 gene from Brassica napus, is required for salinity and osmotic stress-induced lateral root formation.
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DOI：
10.1093/jxb/err190
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发表时间：
2011-08
影响因子：
6.9
通讯作者：
Guan R
中科院分区：
文献类型：
--
作者：
Cao Z;Geng B;Xu S;Xuan W;Nie L;Shen W;Liang Y;Guan R
本文克隆了一个油菜（Brassica napus）血红素加氧酶-1基因BnHO1，并对其进行了测序。它与拟南芥HY1蛋白具有高度同源性，编码32.6 kDa蛋白，具有54个氨基酸的转运肽，预测25.1 kDa的成熟蛋白。在大肠杆菌中成熟表达的BnHO1具有血红素加氧酶（HO）活性。此外，低剂量NaCl （10 mM）和聚乙二醇（PEG）（2%）的处理模拟了萘乙酸和HO-1诱导剂haemin对BnHO1表达上调和随后侧根形成的诱导作用。当NaCl或PEG剂量较高时，观察到对比效果。施用HO-1抑制剂原卟啉锌IX （ZnPPIX）或血红蛋白时，上述诱导和抑制反应被明显阻断，而施用一氧化碳或ZnPPIX时，上述诱导和抑制反应被逆转。此外，在LR形成的不同时间点添加ZnPPIX表明BnHO1可能参与了LR形成的早期阶段。低剂量盐胁迫和渗透胁迫对幼苗根系生长素反应因子转录本和生长素含量有明显的诱导作用。然而，用生长素极性转运抑制剂n -1-萘酞酸处理，抑制了低剂量NaCl和PEG所产生的上述诱导反应，当与血红蛋白联合处理时，这些诱导反应进一步恢复。综上所述，这些结果表明了油菜HO-1基因在盐度和渗透胁迫诱导的LR形成中的新作用，并可能与生长素信号传导相互作用。
In this report, a rapeseed (Brassica napus) haem oxygenase-1 gene BnHO1 was cloned and sequenced. It shared high homology with Arabidopsis HY1 proteins, and encodes a 32.6 kDa protein with a 54-amino-acid transit peptide, predicting the mature protein of 25.1 kDa. The mature BnHO1 expressed in Escherichia coli exhibits haem oxygenase (HO) activity. Furthermore, the application of lower doses of NaCl (10 mM) and polyethylene glycol (PEG) (2%) mimicked the inducible effects of naphthylacetic acid and the HO-1 inducer haemin on the up-regulation of BnHO1 and subsequent lateral root (LR) formation. Contrasting effects were observed when a higher dose of NaCl or PEG was applied. The above inducible and inhibitory responses were blocked significantly when the HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) or haemin was applied, both of which were reversed by the application of carbon monoxide or ZnPPIX, respectively. Moreover, the addition of ZnPPIX at different time points during LR formation indicated that BnHO1 might be involved in the early stages of LR formation. The auxin response factor transcripts and the auxin content in seedling roots were clearly induced by lower doses of salinity or osmotic stress. However, treatment with the inhibitor of polar auxin transport N-1-naphthylphthalamic acid prevented the above inducible responses conferred by lower doses of NaCl and PEG, which were further rescued when the treatments were combined with haemin. Taken together, these results suggested a novel role of the rapeseed HO-1 gene in salinity and osmotic stress-induced LR formation, with a possible interaction with auxin signalling.
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