Nuclear Receptor Interaction Protein (NRIP) expression assay using human tissue microarray and immunohistochemistry technology confirming nuclear localization.
Nuclear Receptor Interaction Protein (NRIP) expression assay using human tissue microarray and immunohistochemistry technology confirming nuclear localization.
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DOI：
10.1186/1756-9966-27-25
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发表时间：
2008-08-02
期刊：
影响因子：
--
通讯作者：
Kok LF
中科院分区：
文献类型：
--
作者：
Han CP;Lee MY;Tzeng SL;Yao CC;Wang PH;Cheng YW;Chen SL;Wu TS;Tyan YS;Kok LF
Chen SL等最近发现了一种新的人核受体相互作用蛋白（NRIP），其可能在增强前列腺癌（LNCaP）和宫颈癌（C33A）细胞系中类固醇核受体的转录活性中起作用。然而，关于NRIP的生物学功能和临床意义的知识仍然不完整。我们的目的是确定NRIP表达的分布，并描绘在各种恶性肿瘤和健康的非病理组织中表达NRIP的细胞类型。这些信息将显著影响其在健康和肿瘤细胞中的生理作用的探索。通过使用组织芯片（TMA）技术和抗NRIP单克隆抗体免疫组化（IHC）的调查，NRIP的表达在48种类型的肿瘤和对照组的48个匹配或不匹配的健康非肿瘤组织进行了检查。我们的调查结果显示，10例显示，在6种恶性肿瘤（食管癌，结肠癌，乳腺癌，卵巢癌，皮肤癌和胰腺癌）表达NRIP，但并非所有这些特定的肿瘤类型始终显示阳性NRIP表达。此外，胃、前列腺、肝、肺、肾、宫颈、膀胱、淋巴结、睾丸和舌的恶性肿瘤均未显示NRIP表达。在48个匹配和不匹配的非肿瘤组织的对照组中，所有这些组织均显示IHC评分小于临界阈值3。此外，36例癌组织中有10例组织核表达NRIP，表明癌组织核中NRIP的表达明显高于正常对照组织。这是第一项使用人TMA和IHC来验证这种新鉴定的NRIP表达的核定位的研究。在考虑使用NRIP作为人类恶性肿瘤调查的潜在诊断工具时，重要的是要注意NRIP表达的敏感性仅为23%，但特异性为100%。原发癌组织与正常对照组织中NRIP的阳性表达也有显著性差异。虽然进一步的大规模研究将值得进行，以评估其作为癌症诊断的潜在辅助作用，本研究的数据提供了有价值的参考，为未来的调查的生物学功能的NRIP在人类。
A novel human nuclear receptor interaction protein (NRIP) has recently been discovered by Chen SL et al, which may play a role in enhancing the transcriptional activity of steroid nuclear receptors in prostate (LNCaP) and cervical (C33A) cancer cell lines. However, knowledge about the biological functions and clinical implications of NRIP, is still incomplete. Our aim was to determine the distribution of NRIP expression and to delineate the cell types that express NRIP in various malignant tumors and healthy non-pathological tissues. This information will significantly affect the exploration of its physiological roles in healthy and tumor cells. By using tissue microarray (TMA) technology and an anti-NRIP monoclonal antibody immunohistochemical (IHC) survey, NRIP expression was examined in 48 types of tumors and in a control group of 48 matched or unmatched healthy non-neoplastic tissues. Our survey results showed that ten cases were revealed to express the NRIP in six malignancies (esophageal, colon, breast, ovarian, skin, and pancreatic cancers), but not all of these specific tumor types consistently showed positive NRIP expression. Moreover, malignant tumors of the stomach, prostate, liver, lung, kidney, uterine cervix, urinary bladder, lymph node, testis, and tongue revealed no NRIP expression. Among the control group of 48 matched and unmatched non-neoplastic tissues, all of them demonstrated IHC scores less than the cut-off threshold of 3. In addition, ten cores out of thirty-six carcinomatous tissues revealed positive NRIP expression, which indicated that NRIP expression increases significantly in carcinoma tissue cores, comparing to the matched controlled healthy tissues. This is the first study to use a human TMA and IHC to validate the nuclear localization for this newly identified NRIP expression. In considering the use of NRIP as a potential diagnostic tool for human malignancies survey, it is important to note that NRIP expression carries a sensitivity of only 23%, but has a specificity of 100%. There is also a significant difference in positive NRIP expression between primary carcinomatous tissues and matched controlled healthy tissues. Although further large-scale studies will merit to be conducted to evaluate its role as a potential adjunct for cancer diagnosis, data from this study provides valuable references for the future investigation of the biological functions of NRIP in humans.
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影响因子：
14.9
作者：
Chen PH;Tsao YP;Wang CC;Chen SL
通讯作者：
Chen SL
DOI：
10.1080/14766650252962649
发表时间：
2002-01-01
期刊：
Journal of cancer epidemiology and prevention
影响因子：
--
作者：
Campbell, H;Holmes, E;Jones, I
通讯作者：
Jones, I
影响因子：
3.4
作者：
PINDER, SE;ELLIS, IO;ELSTON, CW
通讯作者：
ELSTON, CW
影响因子：
4.8
作者：
Tsai, TC;Lee, YL;Chen, SL
通讯作者：
Chen, SL
影响因子：
3.5
作者：
Kallioniemi, OP;Wagner, U;Sauter, G
通讯作者：
Sauter, G