Stability by multimer resolution of pJHCMW1 is due to the Tn1331 resolvase and not to the Escherichia coli Xer system.
Stability by multimer resolution of pJHCMW1 is due to the Tn1331 resolvase and not to the Escherichia coli Xer system.
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pJHCMW1 多聚体解析的稳定性归因于 Tn1331 解析酶，而不是大肠杆菌 Xer 系统。
DOI：
10.1099/00221287-146-3-581
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Sherratt,DavidJ
中科院分区：
文献类型：
--
作者：
Tolmasky,MarceloE;Colloms,Sean;Blakely,Garry;Sherratt,DavidJ
The plasmid pJHCMW1 encodes resistance to several aminoglycosides and β-lactams and consists of a copy of the transposon Tn1331, a region including the replication functions, and a sequence with homology to ColE1cer, designatedmwr. In this work, the role of thiscer-like site in ensuring the stable inheritance of pJHCMW1 by multimer resolution was studied. TheEscherichiacoliXer site-specific recombination system acts at sites such as ColE1certo resolve plasmid multimers formed by homologous recombination, thereby maintaining plasmids in a monomeric state and helping to ensure stable plasmid inheritance. Despite its high similarity to ColE1cer, the pJHCMW1mwrwas a poor substrate for Xer recombination inE. coliand did not contribute significantly to plasmid stability. Instead, the Tn1331co-integrate resolution system was highly active at resolving pJHCMW1 multimers and ensured the stable inheritance of pJHCMW1. Although Xer recombination at pJHCMW1mwrwas inefficient inE. coli, the recombination that did occur was dependent on ArgR, PepA, XerC and XerD. A supercoiled circular DNA molecule containing two pJHCMW1mwrsites in direct repeat yielded Holliday-junction-containing product when incubated with ArgR, PepA, XerC and XerDin vitro, confirming that pJHCMW1mwris a functional recombination site. However, unlikecer, some Holliday-junction-containing product could be detected formwrin the absence of ArgR, although addition of this protein resulted in formation of more Holliday junctions. Binding experiments demonstrated that XerD bound to pJHCMW1mwrcore with a high affinity, but that XerC bound to this site very poorly, even in the presence of XerD.
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[细菌中的染色体分配]。
DOI：
--
发表时间：
1989
期刊：
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
影响因子：
--
作者：
H. Niki;S. Hiraga
通讯作者：
S. Hiraga
ColE1 cer 的衍生物在位点特异性重组中表现出改变的拓扑特异性。
影响因子：
11.4
作者：
D. Summers
通讯作者：
D. Summers
多抗性转座子 Tn1331 的 aadA、bla 和 tnpR 的测序和表达。
影响因子：
2.6
作者：
M. Tolmasky
通讯作者：
M. Tolmasky
影响因子：
11.4
作者：
STIRLING, CJ;COLLOMS, SD;SHERRATT, DJ
通讯作者：
SHERRATT, DJ
影响因子：
4.9
作者：
M. Tolmasky;J. H. Crosa
通讯作者：
M. Tolmasky;J. H. Crosa