l‐Rhamnose catabolism in archaea
l‐Rhamnose catabolism in archaea
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古细菌中的鼠李糖分解代谢
DOI：
10.1111/mmi.14213
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发表时间：
2019
影响因子：
3.6
通讯作者：
Schönheit P.
中科院分区：
文献类型：
--
作者：
Reinhardt A;Johnsen U ;Schönheit P.
嗜盐古生物Haloferax火山利用鼠李糖作为唯一的碳和能源。结果表明，l-鼠李糖被ABC转运蛋白摄取，并通过二酮水解酶途径氧化降解为丙酮酸和l-乳酸。涉及鼠李糖摄取和降解的基因形成了鼠李糖分解代谢(RHC)基因簇。该簇还包含一个基因rhcR，该基因编码转录调节基因RhcR，其特征是激活所有的rhcr基因。2-酮-3-脱氧-L鼠李糖降解中间体2-酮-3-脱氧-L鼠李糖被鉴定为RhcR的诱导分子。基因敲除突变体证实了rh基因在l-鼠李糖的吸收和降解中的重要作用。研究了1-鼠李糖脱氢酶、L-鼠李糖内酯酶、L-鼠李糖酸脱水酶、2-酮-3-脱氧-L-鼠李糖酸脱氢酶和2，4-二酮-3-脱氧-L-鼠李糖酸水解酶。此外，还在嗜热古生菌Vulcanisaeta和Sulfolobus solfararicus中发现了二酮水解酶途径的基因，并对部分酶进行了鉴定，表明这些古菌中存在二酮水解酶途径。总之，这是在古生菌领域首次全面描述l-鼠李糖的分解代谢。
The halophilic archaeonHaloferax volcaniiutilizesl‐rhamnose as a sole carbon and energy source. It is shown thatl‐rhamnose is taken up by an ABC transporter and is oxidatively degraded to pyruvate andl‐lactate via the diketo‐hydrolase pathway. The genes involved inl‐rhamnose uptake and degradation form al‐rhamnose catabolism (rhc) gene cluster. Therhccluster also contains a gene,rhcR, that encodes the transcriptional regulator RhcR which was characterized as an activator of allrhcgenes. 2‐keto‐3‐deoxy‐l‐rhamnonate, a metabolic intermediate ofl‐rhamnose degradation, was identified as inducer molecule of RhcR. The essential function ofrhcgenes for uptake and degradation ofl‐rhamnose was proven by the respective knockout mutants. Enzymes of the diketo‐hydrolase pathway, includingl‐rhamnose dehydrogenase,l‐rhamnonolactonase,l‐rhamnonate dehydratase, 2‐keto‐3‐deoxy‐l‐rhamnonate dehydrogenase and 2,4‐diketo‐3‐deoxy‐l‐rhamnonate hydrolase, were characterized. Further, genes of the diketo‐hydrolase pathway were also identified in the hyperthermophilic crenarchaeotaVulcanisaeta distributaandSulfolobus solfataricusand selected enzymes were characterized, indicating the presence of the diketo‐hydrolase pathway in these archaea. Together, this is the first comprehensive description ofl‐rhamnose catabolism in the domain of archaea.
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影响因子：
4.1
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