Immunological and pathological outcomes of SARS-CoV-2 challenge following formalin-inactivated vaccine in ferrets and rhesus macaques.
Immunological and pathological outcomes of SARS-CoV-2 challenge following formalin-inactivated vaccine in ferrets and rhesus macaques.
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DOI：
10.1126/sciadv.abg7996
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发表时间：
2021-09-10
期刊：
影响因子：
13.6
通讯作者：
Carroll M
中科院分区：
文献类型：
--
作者：
Bewley KR;Gooch K;Thomas KM;Longet S;Wiblin N;Hunter L;Chan K;Brown P;Russell RA;Ho C;Slack G;Humphries HE;Alden L;Allen L;Aram M;Baker N;Brunt E;Cobb R;Fotheringham S;Harris D;Kennard C;Leung S;Ryan K;Tolley H;Wand N;White A;Sibley L;Sarfas C;Pearson G;Rayner E;Xue X;Lambe T;Charlton S;Gilbert S;Sattentau QJ;Gleeson F;Hall Y;Funnell S;Sharpe S;Salguero FJ;Gorringe A;Carroll M
在雪貂或恒河猴中未观察到灭活SARS-CoV-2疫苗后疫苗增强型疾病的证据。目前迫切需要安全有效的疫苗来预防COVID-19。开发新病毒疫苗的一个令人关切的问题是可能诱发疫苗增强型疾病。在几项针对SARS-CoV-1和MERS疫苗的临床前研究中都报道了这一点，但没有针对SARS-CoV-2疫苗的报道。我们使用了受到SARS-CoV-2攻击的雪貂和恒河猴，以评估与阴性对照疫苗相比，接种了醛水凝胶配制的甲醛灭活SARS-CoV-2 （FIV）疫苗的动物发生VED的可能性。我们没有发现在雪貂或恒河猴感染FIV后，除了在雪貂感染后7天出现轻微的短暂性疾病增强外，疾病增强的证据。在第7天观察到这种肺部病理增加，但在第15天消失。我们还证明，SARS-CoV-2的甲醛处理减少了刺突受体结合域的暴露，这为次优免疫提供了机制解释。
No evidence of vaccine-enhanced disease was observed following inactivated SARS-CoV-2 vaccine in ferrets or rhesus macaques. There is an urgent requirement for safe and effective vaccines to prevent COVID-19. A concern for the development of new viral vaccines is the potential to induce vaccine-enhanced disease (VED). This was reported in several preclinical studies with both SARS-CoV-1 and MERS vaccines but has not been reported with SARS-CoV-2 vaccines. We have used ferrets and rhesus macaques challenged with SARS-CoV-2 to assess the potential for VED in animals vaccinated with formaldehyde-inactivated SARS-CoV-2 (FIV) formulated with Alhydrogel, compared to a negative control vaccine. We showed no evidence of enhanced disease in ferrets or rhesus macaques given FIV except for mild transient enhanced disease seen 7 days after infection in ferrets. This increased lung pathology was observed at day 7 but was resolved by day 15. We also demonstrate that formaldehyde treatment of SARS-CoV-2 reduces exposure of the spike receptor binding domain providing a mechanistic explanation for suboptimal immunity.
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DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
影响因子：
64.8
作者：
Arvin, Ann M.;Fink, Katja;Virgin, Herbert W.
通讯作者：
Virgin, Herbert W.
影响因子：
11.4
作者：
Caly, Leon;Druce, Julian;Catton, Mike G.
通讯作者：
Catton, Mike G.
DOI：
10.1126/science.aan6836
发表时间：
2017-11-17
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Katzelnick LC;Gresh L;Halloran ME;Mercado JC;Kuan G;Gordon A;Balmaseda A;Harris E
通讯作者：
Harris E
影响因子：
16.6
作者：
Guttman M;Cupo A;Julien JP;Sanders RW;Wilson IA;Moore JP;Lee KK
通讯作者：
Lee KK