Activation and deactivation of DNAzyme and antisense function with light for the photochemical regulation of gene expression in mammalian cells.
Activation and deactivation of DNAzyme and antisense function with light for the photochemical regulation of gene expression in mammalian cells.
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DOI：
10.1021/ja100710j
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发表时间：
2010-05-05
影响因子：
15
通讯作者：
Deiters A
中科院分区：
文献类型：
--
作者：
Young DD;Lively MO;Deiters A
生物系统的光化学调控是一种非常精确的方法，可以在空间和时间上实现对基因表达的高分辨率控制。DNAzymes是具有酶活性的脱氧寡核苷酸，能够实现RNA的位点特异性切割，并已被用于各种体外应用。我们之前已经报道了通过制备笼状DNA磷酰胺及其位点特异性结合到寡核苷酸中的DNAzymes和反义剂的光化学激活。笼化基团的存在会破坏DNA:RNA杂交或催化活性，直到用紫外线短暂照射去除。在这里，我们通过研究DNAzymes和反义剂的光化学失活来扩展这一概念。此外，我们还报道了光激活和光失活反义药物在哺乳动物细胞基因功能调控中的应用。这代表了在哺乳动物组织培养中可以开启和关闭基因沉默反义因子的第一个例子。
The photochemical regulation of biological systems represents a very precise means of achieving high-resolution control over gene expression in both a spatial and a temporal fashion. DNAzymes are enzymatically active deoxyoligonucleotides that enable the site-specific cleavage of RNA, and have been used in a variety of in vitro applications. We have previously reported the photochemical activation of DNAzymes and antisense agents through the preparation of a caged DNA phosphoramidite and its site-specific incorporation into oligonucleotides. The presence of the caging group disrupts either DNA:RNA hybridization or catalytic activity, until removed via a brief irradiation with UV light. Here, we are expanding this concept by investigating the photochemical deactivation of DNAzymes and antisense agents. Moreover, we report the application of light-activated and light-deactivated antisense agents to the regulation of gene function in mammalian cells. This represents the first example of gene silencing antisense agents that can be turned on and turned off in mammalian tissue culture.
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影响因子：
3.5
作者：
Abdelgany, Amr;Wood, Matthew;Beeson, David
通讯作者：
Beeson, David
DOI：
10.1007/s00018-008-8029-y
发表时间：
2008-07
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Baum DA;Silverman SK
通讯作者：
Silverman SK
影响因子：
--
作者：
Blidner, Richard A.;Svoboda, Kurt R.;Monroe, W. Todd
通讯作者：
Monroe, W. Todd
影响因子：
5.2
作者：
Lusic, Hrvoje;Young, Douglas D.;Deiters, Alexander
通讯作者：
Deiters, Alexander
影响因子：
3.2
作者：
Deiters, Alexander
通讯作者：
Deiters, Alexander