Chemogenetic selective manipulation of nucleus accumbens medium spiny neurons bidirectionally controls alcohol intake in male and female rats.
Chemogenetic selective manipulation of nucleus accumbens medium spiny neurons bidirectionally controls alcohol intake in male and female rats.
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DOI：
10.1038/s41598-020-76183-2
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发表时间：
2020-11-05
影响因子：
4.6
通讯作者：
Kabbaj M
中科院分区：
文献类型：
--
作者：
Strong CE;Hagarty DP;Brea Guerrero A;Schoepfer KJ;Cajuste SM;Kabbaj M
The nucleus accumbens (NAc), considered the hub of reward circuitry, is comprised of two medium spiny neuron (MSN) subtypes that are classified by their enrichment of dopamine 1 (D1) or 2 (D2) receptors. While reports indicate that alcohol increases excitatory neurotransmission exclusively on NAc D1-MSNs in male rats, it remains unknown how NAc MSNs control alcohol intake in either sex. Therefore, this study investigated how NAc MSNs mediate alcohol intake by using Drd1a-iCre and Drd2-iCre transgenic rats of both sexes. Intra-NAc infusions of Cre-inducible viral vectors containing stimulatory (hM3Dq) or inhibitory (hM4Di) designer receptors exclusively activated by designer drugs (DREADDs) were delivered after 4-weeks of alcohol intake, and clozapine-N-oxide (CNO) was administered to selectively manipulate NAc MSNs. Our results show that activation of NAc D1-MSNs increased alcohol intake 1-, 4-, and 24-h after the start of drinking while inhibition decreased it 1-h after the start of drinking, with no sex differences observed at any time point. Activation of NAc D2-MSNs had no impact on alcohol intake while inhibition increased alcohol intake in Drd2-iCre rats for 1-h in males and 4-h in females. These findings suggest opposing roles for how NAc D1- and D2-MSNs modulate alcohol intake in rats of both sexes.
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影响因子：
3.3
作者：
Hauser SR;Deehan GA Jr;Dhaher R;Knight CP;Wilden JA;McBride WJ;Rodd ZA
通讯作者：
Rodd ZA
影响因子：
16.2
作者：
Creed M;Ntamati NR;Chandra R;Lobo MK;Lüscher C
通讯作者：
Lüscher C
影响因子：
7.6
作者：
Czoty, Paul W.;Riddick, Natallia V.;Nader, Michael A.
通讯作者：
Nader, Michael A.
DOI：
10.5350/dajpn2010230203t
发表时间：
2010-06-01
影响因子：
1
作者：
Evren, Cuneyt;Durkaya, Mine;Cakmak, Duran
通讯作者：
Cakmak, Duran
影响因子：
15.9
作者：
Krashes, Michael J.;Koda, Shuichi;Lowell, Bradford B.
通讯作者：
Lowell, Bradford B.