First Evidence for the Existence of Multiple Isoforms of Bovine Serum Amyloid‐A (apoSAA)
First Evidence for the Existence of Multiple Isoforms of Bovine Serum Amyloid‐A (apoSAA)
复制标题
牛血清淀粉样蛋白-A (apoSAA) 多种亚型存在的第一个证据
DOI:
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发表时间:
1995
影响因子:
3.7
通讯作者:
Erik Gruys
中科院分区:
文献类型:
--
作者:
S. Alsemgeest;A. Horadagoda;C. Hulskamp;P. Tooten;D. H. Kim;Theo A. Niewold;Erik Gruys
Bovine serum amyloid‐A (SAA) was purified from acute‐phase high density iipoprotein (HDL) by affinity chromatography and subsequent gel filtration chromatography. The identity of the isolated protein was checked by Western blotting following SDS‐urea‐PAGE using antisera raised against the purified protein fraction (SAA) and Amyloid A (AA). The antiserum raised against the purified SAA stained Congo red positive regions in the kidney of an AA‐amyloidotic cow and reacted on Western blot with an AA‐related protein of approximately 14kDa. Moreover, it immunostained two to three bands, of approximately 14kDa, present in serum from diseased cows, proportionally to the serum SAA concentration as measured by ELISA.
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DOI:
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发表时间:
1982
期刊:
Journal of immunology (Baltimore, Md. : 1950)
影响因子:
--
作者:
Tobias,PS;McAdam,KP;Ulevitch,RJ
通讯作者:
Ulevitch,RJ
影响因子:
56.9
作者:
BRINCKERHOFF, CE;MITCHELL, TI;BENSON, MD
通讯作者:
BENSON, MD
DOI:
--
发表时间:
1981
期刊:
Laboratory investigation; a journal of technical methods and pathology
影响因子:
--
作者:
Doepel,FM;Glorioso,JC;Newcomer,CE;Skinner,M;Abrams,GD
通讯作者:
Abrams,GD
DOI:
--
发表时间:
1989
期刊:
The Journal of laboratory and clinical medicine
影响因子:
--
作者:
Benson,MD;DiBartola,SP;Dwulet,FE
通讯作者:
Dwulet,FE