SARS-CoV-2 variants of concern partially escape humoral but not T-cell responses in COVID-19 convalescent donors and vaccinees.
SARS-CoV-2 variants of concern partially escape humoral but not T-cell responses in COVID-19 convalescent donors and vaccinees.
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DOI：
10.1126/sciimmunol.abj1750
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发表时间：
2021-05-25
影响因子：
24.8
通讯作者：
中科院分区：
文献类型：
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作者：
感染或疫苗接种诱导的 SARS-CoV-2 S 特异性 CD4+ T 细胞对相关变体的 S 突变无关。带有刺突 (S) 蛋白突变的 SARS-CoV-2 变体的出现引起了人们对潜在免疫逃逸的担忧。在这里，我们在 121 名接种过 BNT162b2 信使 RNA 疫苗的医护人员 (HCW) 中研究了对野生型 SARS-CoV-2 以及相关 B.1.1.7 和 B.1.351 变体的体液和细胞免疫反应。 23 名医护人员从轻度 COVID-19 疾病中康复，并在单次疫苗接种后表现出高水平的 SARS-CoV-2 特异性功能抗体和病毒特异性 T 细胞的回忆反应。接种一次疫苗后，在血清阴性医护人员中也检测到了特异性免疫反应，但需要第二剂才能在所有个体中达到高水平的功能性抗体和细胞免疫反应。疫苗诱导的抗体可交叉中和变体 B.1.1.7 和 B.1.351，但针对 B.1.351 的中和能力和 Fc 介导的功能始终比针对同源病毒的中和能力低两到四倍。此外，使用跨越 B.1.1.7 和 B.1.351 突变 S 区的肽池刺激外周血单核细胞，以检测 SARS-CoV-2 特异性 T 细胞与变异体的交叉反应性。我们观察到 CD4+ T 细胞激活对变异抗原的反应没有差异，表明 B.1.1.7 和 B.1.351 S 蛋白不会逃避野生型 S 蛋白引发的 T 细胞介导的免疫。总之，本研究表明，一些变体可以部分逃避由 SARS-CoV-2 感染或 BNT162b2 疫苗接种诱导的体液免疫，但 S 特异性 CD4+ T 细胞激活不受 B.1.1.7 和 B.1.351 变体突变的影响。
Infection- or vaccination-induced SARS-CoV-2 S–specific CD4+ T cells are indifferent to the S mutations of variants of concern. The emergence of SARS-CoV-2 variants harboring mutations in the spike (S) protein has raised concern about potential immune escape. Here, we studied humoral and cellular immune responses to wild-type SARS-CoV-2 and the B.1.1.7 and B.1.351 variants of concern in a cohort of 121 BNT162b2 messenger RNA–vaccinated health care workers (HCWs). Twenty-three HCWs recovered from mild COVID-19 disease and exhibited a recall response with high levels of SARS-CoV-2–specific functional antibodies and virus-specific T cells after a single vaccination. Specific immune responses were also detected in seronegative HCWs after one vaccination, but a second dose was required to reach high levels of functional antibodies and cellular immune responses in all individuals. Vaccination-induced antibodies cross-neutralized the variants B.1.1.7 and B.1.351, but the neutralizing capacity and Fc-mediated functionality against B.1.351 were consistently two- to fourfold lower than those against the homologous virus. In addition, peripheral blood mononuclear cells were stimulated with peptide pools spanning the mutated S regions of B.1.1.7 and B.1.351 to detect cross-reactivity of SARS-CoV-2–specific T cells with variants. We observed no differences in CD4+ T cell activation in response to variant antigens, indicating that the B.1.1.7 and B.1.351 S proteins do not escape T cell–mediated immunity elicited by the wild-type S protein. In conclusion, this study shows that some variants can partially escape humoral immunity induced by SARS-CoV-2 infection or BNT162b2 vaccination, but S-specific CD4+ T cell activation is not affected by the mutations in the B.1.1.7 and B.1.351 variants.
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影响因子：
64.8
作者：
Gaebler C;Wang Z;Lorenzi JCC;Muecksch F;Finkin S;Tokuyama M;Cho A;Jankovic M;Schaefer-Babajew D;Oliveira TY;Cipolla M;Viant C;Barnes CO;Bram Y;Breton G;Hägglöf T;Mendoza P;Hurley A;Turroja M;Gordon K;Millard KG;Ramos V;Schmidt F;Weisblum Y;Jha D;Tankelevich M;Martinez-Delgado G;Yee J;Patel R;Dizon J;Unson-O'Brien C;Shimeliovich I;Robbiani DF;Zhao Z;Gazumyan A;Schwartz RE;Hatziioannou T;Bjorkman PJ;Mehandru S;Bieniasz PD;Caskey M;Nussenzweig MC
通讯作者：
Nussenzweig MC
DOI：
10.1093/cid/ciab172
发表时间：
2021-12-16
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
den Hartog G;Vos ERA;van den Hoogen LL;van Boven M;Schepp RM;Smits G;van Vliet J;Woudstra L;Wijmenga-Monsuur AJ;van Hagen CCE;Sanders EAM;de Melker HE;van der Klis FRM;van Binnendijk RS
通讯作者：
van Binnendijk RS
影响因子：
64.5
作者：
Hoffmann M;Arora P;Groß R;Seidel A;Hörnich BF;Hahn AS;Krüger N;Graichen L;Hofmann-Winkler H;Kempf A;Winkler MS;Schulz S;Jäck HM;Jahrsdörfer B;Schrezenmeier H;Müller M;Kleger A;Münch J;Pöhlmann S
通讯作者：
Pöhlmann S
影响因子：
15.9
作者：
Gong, Fang;Dai, Yaping;Zhou, Pengcheng
通讯作者：
Zhou, Pengcheng
DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group