Global DNA methylation variations after short-term heat shock treatment in cultured microspores of Brassica napus cv. Topas.
Global DNA methylation variations after short-term heat shock treatment in cultured microspores of Brassica napus cv. Topas.
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DOI：
10.1038/srep38401
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发表时间：
2016-12-05
影响因子：
4.6
通讯作者：
Wu X
中科院分区：
文献类型：
--
作者：
Li J;Huang Q;Sun M;Zhang T;Li H;Chen B;Xu K;Gao G;Li F;Yan G;Qiao J;Cai Y;Wu X
热胁迫可诱导小孢子胚状体发生。利用全基因组亚硫酸氢盐测序技术，研究了甘蓝型油菜小孢子经短期热激处理后DNA甲基化的变化。托帕斯我们的研究结果表明，在32 °C下处理培养的Topas小孢子6小时触发DNA低甲基化，特别是在CG和CHG背景下。T32（Topas 32 °C处理6 h）与T0（Topas 0 h）差异甲基化区域相关基因（DRGs）的总数比T18（Topas 18 °C处理6 h）与T0 DRGs的总数高约2倍，这表明32 °C可能是比18 °C更强烈的外部刺激，导致培养小孢子DNA甲基化状态发生更多变化。此外，32 °C处理6 h导致转座子（DMT）的CHG差异甲基化增加，其主要由低甲基化差异甲基化区域（hypo-DMR）和转座子元件（TE）之间的重叠构成。进一步的分析表明，背根神经节及其旁系同源物表现出不同的甲基化/去甲基化模式。总之，本研究是第一个甲基化组分析培养的小孢子在响应STHS，并可能提供有价值的信息的作用，DNA甲基化在热反应。
Heat stress can induce the cultured microspores into embryogenesis. In this study, whole genome bisulphite sequencing was employed to study global DNA methylation variations after short-term heat shock (STHS) treatments in cultured microspores of Brassica napus cv. Topas. Our results indicated that treatment on cultured Topas microspores at 32 °C for 6 h triggered DNA hypomethylation, particularly in the CG and CHG contexts. And the total number of T32 (Topas 32 °C for 6 h) vs. T0 (Topas 0 h) differentially methylated region-related genes (DRGs) was approximately two-fold higher than that of T18 (Topas 18 °C for 6 h) vs. T0 DRGs, which suggested that 32 °C might be a more intense external stimulus than 18 °C resulting in more changes in the DNA methylation status of cultured microspores. Additionally, 32 °C treatment for 6 h led to increased CHG differential methylations of transposons (DMTs), which were mainly constituted by overlaps between the hypomethylated differentially methylated regions (hypo-DMRs) and transposon elements (TEs). Further analysis demonstrated that the DRGs and their paralogs exhibited differential methylated/demethylated patterns. To summarize, the present study is the first methylome analysis of cultured microspores in response to STHS and may provide valuable information on the roles of DNA methylation in heat response.
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影响因子：
3.7
作者：
Boyko A;Blevins T;Yao Y;Golubov A;Bilichak A;Ilnytskyy Y;Hollunder J;Meins F Jr;Kovalchuk I
通讯作者：
Kovalchuk I
影响因子：
5.6
作者：
Kavi Kishor PB;Hima Kumari P;Sunita MS;Sreenivasulu N
通讯作者：
Sreenivasulu N
影响因子：
2.9
作者：
Gervais, C;Newcomb, W;Simmonds, DH
通讯作者：
Simmonds, DH
影响因子：
5.6
作者：
Liu J;Feng L;Li J;He Z
通讯作者：
He Z
DOI：
10.1093/dnares/dsm013
发表时间：
2007-06-30
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Arnaud D;Déjardin A;Leplé JC;Lesage-Descauses MC;Pilate G
通讯作者：
Pilate G