The molecular characterization of bovine leukaemia virus isolates from Eastern Europe and Siberia and its impact on phylogeny.
The molecular characterization of bovine leukaemia virus isolates from Eastern Europe and Siberia and its impact on phylogeny.
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DOI：
10.1371/journal.pone.0058705
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kuźmak J
中科院分区：
文献类型：
--
作者：
Rola-Łuszczak M;Pluta A;Olech M;Donnik I;Petropavlovskiy M;Gerilovych A;Vinogradova I;Choudhury B;Kuźmak J
最近的研究表明，牛白血病病毒（BLV）可以分为七个不同的基因型的基础上，完整的gp51序列。该分类基于可用的序列数据，主要代表在美国和南美洲牛中传播的BLV群体。为了从全球角度提供帮助，需要纳入东欧的数据。在这项研究中，我们检查了来自波兰，白俄罗斯，乌克兰和俄罗斯的不同地理区域的44个BLV分离株。基于env基因444bp片段的系统发育分析表明，绝大多数分离物属于基因型4和7。此外，我们证实了一个新的基因型，基因型8，这是高度支持的系统发育计算。在研究的东欧分离株的序列中发现了大量的氨基酸取代，其中71%以前没有描述过。这些取代主要包括CD4+表位的C部分、锌结合肽区、CD8+ T细胞表位和重叠线性表位E。这些观察结果强调了使用序列数据来阐明系统发育关系和对地理上不同分离株的血清学检测的潜在影响。
Recent studies have shown that bovine leukemia virus (BLV) sequences can be classified into seven distinct genotypes based on full gp51 sequence. This classification was based on available sequence data that mainly represented the BLV population that is circulating in cattle from the US and South America. In order to aid with a global perspective inclusion of data from Eastern Europe is required. In this study we examined 44 BLV isolates from different geographical regions of Poland, Belarus, Ukraine, and Russia. Phylogenetic analysis based on a 444bp fragment of env gene revealed that most of isolates belonged to genotypes 4 and 7. Furthermore, we confirmed the existence of a new genotype, genotype 8, which was highly supported by phylogenetic computations. A significant number of amino acid substitutions were found in the sequences of the studied Eastern European isolates, of which 71% have not been described previously. The substitutions encompassed mainly the C-part of the CD4+ epitope, zinc binding peptide region, CD8+ T cell epitope, and overlapping linear epitope E. These observations highlight the use of sequence data to both elucidate phylogenetic relationships and the potential effect on serological detection of geographically diverse isolates.
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DOI：
10.1046/j.1439-0450.2002.00582.x
发表时间：
2002-09-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Camargos, MF;Stancek, D;Leite, RC
通讯作者：
Leite, RC
影响因子：
2.2
作者：
Hemmatzadeh, F.
通讯作者：
Hemmatzadeh, F.
影响因子：
5.6
作者：
Hall, BG;Barlow, M
通讯作者：
Barlow, M
影响因子：
5.4
作者：
MAMOUN, RZ;MORISSON, M;GUILLEMAIN, B
通讯作者：
GUILLEMAIN, B
影响因子：
5.4
作者：
Johnston, ER;Albritton, LM;Radke, K
通讯作者：
Radke, K