Use of 8-methoxypsoralen and ultraviolet-A pretreated platelet concentrates to prevent alloimmunization against class I major histocompatibility antigens
Use of 8-methoxypsoralen and ultraviolet-A pretreated platelet concentrates to prevent alloimmunization against class I major histocompatibility antigens
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使用 8-甲氧基补骨脂素和紫外线 A 预处理的血小板浓缩物预防针对 I 类主要组织相容性抗原的同种免疫
DOI：
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
K. Kao
中科院分区：
文献类型：
--
作者：
N. Grana;K. Kao
用8-甲氧补骨脂素(8-MOP)和UV-A照射灭活浓缩血小板中受污染的供体白细胞，防止供体I类主要组织相容性(MHC)抗原的初次免疫。分别以H_2k单倍型CBA/CAH-T6J小鼠和H_2d单倍型BALB/cByJ小鼠为供体和受体。两种小鼠的混合白细胞反应表明，500 ng/mL8-MOP和5J/cm2 UV-A处理的脾细胞抑制了99%的应答和92%的刺激功能。治疗后未见明显的血小板聚集活性丧失。在每周两次不经任何处理的血小板输注后，93%的对照组小鼠(n=15)产生了抗H_2k抗体。相比之下，接受8-MOP和UV-A治疗的小鼠中，只有33%的小鼠(n=15)获得了同种异体免疫。在每周六次的血小板输注后，所有的小鼠都获得了同种免疫。但治疗组小鼠血清中抗H_2k抗体平均滴度显著低于对照组。在输注处理过的血小板的小鼠中，51Cr标记的供者血小板在输血后1小时的回收率也更高。因此，用8-MOP和UV-A辐射对浓缩的血小板进行预处理，有效地降低了I类MHC分子的同种异体抗原性。这一发现的含义与供者白细胞使受者同种异体致敏的机制有关进行了讨论。
The use of 8-methoxypsoralen (8-MOP) and UV-A irradiation to inactivate contaminating donor leukocytes in platelet concentrates and to prevent primary alloimmunization against donor class I major histocompatibility (MHC) antigens in mice was investigated. CBA/CaH-T6J mice with the H2k haplotype and BALB/cByJ mice with the H2d haplotype were used as donors and recipients, respectively. The mixed leukocyte reaction between these two strains of mice showed that treatment of spleen cells with 500 ng/mL 8-MOP and 5J/cm2 UV-A inhibited 99% of responder and 92% of stimulator function. There was no measurable loss of platelet aggregating activity after the treatment. After two weekly transfusions of platelets without any treatment, 93% of control mice (n = 15) developed anti-H2k antibody. In contrast, only 33% of mice (n = 15) receiving platelets treated with 8-MOP and UV-A became alloimmunized. After six weekly platelet transfusions, all mice became alloimmunized. Nevertheless, the mean titers of anti-H2k antibody in sera of the treated groups were significantly lower than the control groups. One hour posttransfusion recoveries of 51Cr-labeled donor platelets were also higher in mice transfused with the treated platelets. Thus, the pretreatment of platelet concentrates with 8-MOP and UV-A irradiation effectively reduced the alloantigenicity of class I MHC molecules. The implication of this finding in relation to the mechanism by which donor leukocytes allosensitize recipients is discussed.
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影响因子：
2.9
作者：
Deeg,HJ
通讯作者：
Deeg,HJ
T辅助细胞之间的异质性。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Powers,GD;Miller,RA
通讯作者：
Miller,RA
影响因子：
158.5
作者：
EDELSON, R;BERGER, C;LAROCHE, L
通讯作者：
LAROCHE, L
HLA 表型与血浆或血小板中 HLA 浓度之间的关联。
影响因子：
2.7
作者：
Kao,KJ;Scornik,JC;Riley,WJ;McQueen,CF
通讯作者：
McQueen,CF