Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions.
Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions.
复制标题
适应极端氧气条件的果蝇线粒体蛋白质组的定量评估。
DOI：
10.1371/journal.pone.0074011
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Yin S;Xue J;Sun H;Wen B;Wang Q;Perkins G;Zhao HW;Ellisman MH;Hsiao YH;Yin L;Xie Y;Hou G;Zi J;Lin L;Haddad GG;Zhou D;Liu S
线粒体是消耗氧气并为细胞活动提供能量的主要细胞器。为了研究线粒体对极端氧气条件的适应机制，我们培育了能够在低氧或高氧环境(分别为LOF和HOF)中生存的果蝇品系，通过透射电子显微镜在超微结构水平上观察它们的线粒体，研究它们的呼吸链复合体的活性，并使用相对和绝对定量等压标签(ITRAQ)定量分析线粒体蛋白质组的蛋白质丰度响应。共鉴定出718个蛋白质，其中55个和75个线粒体蛋白质在LOF和HOF中的丰度分别与对照果蝇有显著差异。重要的是，这些差异表达的线粒体蛋白主要参与呼吸、钙调节、氧化反应和线粒体蛋白翻译。对与差异调控线粒体蛋白相对应的mRNAs水平变化的相关性分析表明，LOF和HOF中有两组不同调控模式的蛋白质(转录和转录后)。我们相信，这些发现不仅将加深我们对果蝇适应极端氧气条件的潜在机制的理解，而且还将为研究组织和细胞中氧张力变化引起的人类疾病提供线索。
Mitochondria are the primary organelles that consume oxygen and provide energy for cellular activities. To investigate the mitochondrial mechanisms underlying adaptation to extreme oxygen conditions, we generated Drosophila strains that could survive in low- or high-oxygen environments (LOF or HOF, respectively), examined their mitochondria at the ultrastructural level via transmission electron microscopy, studied the activity of their respiratory chain complexes, and quantitatively analyzed the protein abundance responses of the mitochondrial proteomes using Isobaric tag for relative and absolute quantitation (iTRAQ). A total of 718 proteins were identified with high confidence, and 55 and 75 mitochondrial proteins displayed significant differences in abundance in LOF and HOF, respectively, compared with the control flies. Importantly, these differentially expressed mitochondrial proteins are primarily involved in respiration, calcium regulation, the oxidative response, and mitochondrial protein translation. A correlation analysis of the changes in the levels of the mRNAs corresponding to differentially regulated mitochondrial proteins revealed two sets of proteins with different modes of regulation (transcriptional vs. post-transcriptional) in both LOF and HOF. We believe that these findings will not only enhance our understanding of the mechanisms underlying adaptation to extreme oxygen conditions in Drosophila but also provide a clue in studying human disease induced by altered oxygen tension in tissues and cells.
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影响因子：
2.9
作者：
Glancy, Brian;Balaban, Robert S.
通讯作者：
Balaban, Robert S.
影响因子：
4
作者：
Gyorgy Hajnoczky;Gyrogy Csordas;Yi, Muqing
通讯作者：
Yi, Muqing
影响因子：
3.4
作者：
Aggarwal, K;Choe, LH;Lee, KH
通讯作者：
Lee, KH
影响因子：
7.4
作者：
Hardt, M;Witkowska, HE;Fisher, SJ
通讯作者：
Fisher, SJ
DOI：
10.1073/pnas.91.25.12248
发表时间：
1994-12-06
影响因子：
11.1
作者：
GARDNER, PR;NGUYEN, DDH;WHITE, CW
通讯作者：
WHITE, CW