Varicella-Zoster Virus (VZV) ORF17 Protein Induces RNA Cleavage and Is Critical for Replication of VZV at 37oC but Not 33oC
Varicella-Zoster Virus (VZV) ORF17 Protein Induces RNA Cleavage and Is Critical for Replication of VZV at 37oC but Not 33oC
复制标题
水痘带状疱疹病毒 (VZV) ORF17 蛋白可诱导 RNA 裂解，并且对 VZV 在 37°C（而非 33°C）下的复制至关重要
作者：
Hitoshi Sato;L. Callanan;L. Pesnicak;T. Krogmann;J. Cohen
水痘带状疱疹病毒（VZV）开放阅读框17 （ORF17）与单纯疱疹病毒（HSV） UL41同源，后者编码病毒宿主关闭蛋白（vhs）。HSV vhs诱导mRNA的降解和宿主蛋白合成的快速关闭。ORF17蛋白抗体在vzv感染细胞中检测到46-kDa蛋白。虽然HSV vhs位于病毒粒子中，但VZV ORF17蛋白在病毒粒子中未检测到。ORF17蛋白诱导RNA裂解，但程度远低于HSV-1 vhs。ORF17蛋白的表达不抑制β-半乳糖苷酶报告质粒的表达，而HSV 1型vhs则抑制报告蛋白的表达。构建了两个VZV ORF17缺失突变体，以检测ORF17在病毒复制中的作用。ORF17 VZV突变体在33°C时的峰值滴度与亲本病毒相似，而在37°C时，ORF17突变体的滴度比亲本病毒低20至35倍。ORF62蛋白在37°C与33°C感染ORF17缺失突变体的细胞的细胞核和细胞质中的分布模式不同。用ORF17缺失突变体接种棉花大鼠产生的潜伏感染水平与用亲本病毒接种产生的潜伏感染水平相似。ORF17蛋白在37℃而非33℃条件下对病毒复制的重要性表明该蛋白可能促进病毒在体内某些组织中的生长。
ABSTRACT Varicella-zoster virus (VZV) open reading frame 17 (ORF17) is homologous to herpes simplex virus (HSV) UL41, which encodes the viral host shutoff protein (vhs). HSV vhs induces degradation of mRNA and rapid shutoff of host protein synthesis. An antibody to ORF17 protein detected a 46-kDa protein in VZV-infected cells. While HSV vhs is located in virions, VZV ORF17 protein was not detectable in virions. ORF17 protein induced RNA cleavage, but to a substantially lesser extent than HSV-1 vhs. Expression of ORF17 protein did not inhibit expression from a β-galactosidase reporter plasmid, while HSV type 1 vhs abolished reporter expression. Two VZV ORF17 deletion mutants were constructed to examine the role of ORF17 in virus replication. While the ORF17 VZV mutants grew to peak titers that were similar to those of the parental virus at 33°C, the ORF17 mutants grew to 20- to 35-fold-lower titers than parental virus at 37°C. ORF62 protein was distributed in a different pattern in the nuclei and cytoplasm of cells infected with an ORF17 deletion mutant at 37°C compared to 33°C. Inoculation of cotton rats with the ORF17 deletion mutant resulted in a level of latent infection similar to that produced by inoculation with the parental virus. The importance of ORF17 protein for viral replication at 37°C but not at 33°C suggests that this protein may facilitate the growth of virus in certain tissues in vivo.
使用体外 mRNA 衰减系统分析单纯疱疹病毒诱导的 mRNA 不稳定活性。
影响因子：
14.9
作者：
Sorenson,CM;Hart,PA;Ross,J
通讯作者：
Ross,J
单纯疱疹病毒的病毒体宿主关闭蛋白在没有其他病毒基因产物的情况下抑制报告基因表达。
DOI：
10.1006/viro.1995.1431
发表时间：
1995
期刊：
Virology.
影响因子：
--
作者：
Pak,AS;Everly,DN;Knight,K;Read,GS
通讯作者：
Read,GS