Combining single-molecule sequencing and next-generation sequencing to provide insight into the complex response of Iris halophila Pall. to Pb exposure
Combining single-molecule sequencing and next-generation sequencing to provide insight into the complex response of Iris halophila Pall. to Pb exposure
复制标题
结合单分子测序和新一代测序，深入了解 Iris halophila Pall 的复杂反应。
DOI：
10.1016/j.indcrop.2021.113623
复制
发表时间：
2021-09
影响因子：
5.9
通讯作者：
Haiyan Yuan
中科院分区：
文献类型：
--
作者：
Qingquan Liu;Yongxia Zhang;Yinjie Wang;Chunsun Gu;Suzhen Huang;Om Parkash Dhankher;Haiyan Yuan
蝴蝶花是一种具有很高生态和工业价值的富铅植物，在铅污染土壤的植物修复中具有巨大的应用潜力。然而，缺乏全长转录组信息阻碍了对分子调控网络OFI的理解。嗜盐植物对铅的反应。构建全长转录本数据库，鉴定其分子机制。嗜盐植物对铅的响应，地上部、根、花和铅处理的幼苗。对嗜盐菌进行了单分子实时测序和下一代测序。共预测了40034个完整的编码序列，2028个选择性剪接，4649个长的非编码RNA(LncRNAs)。其中，铅处理1共鉴定出2614个注释差异表达基因(DEG)和19个差异表达的LncRNAs(DELs)。在根中发现了7242个注释DEGS和57个DELs。生物功能丰富分析揭示了激素信号转导、谷胱甘肽代谢、金属离子转运蛋白的关键作用。嗜盐植物对铅的反应。LncRNA在体内起着至关重要的作用。嗜盐植物对铅的抗性通过调节下游的关键代谢途径来实现。这些结果不仅丰富了虹膜的遗传信息。L对植物的研究，也提高了对铅尼分子响应的认识。为今后铅植物修复或低铅粮食作物的研究和发展奠定了重要的基础。工业用嗜盐菌。
Iris halophilaPall., a Pb accumulator with high ecological and industrial values, has enormous application potential for the phytoremediation of Pb-contaminated soil. However, the lack of full-length transcriptome information obstructs understanding of the molecular regulatory network ofI. halophilaresponse to Pb. To construct the full-length transcript database and identify the molecular mechanisms underlyingI. halophilaresponse to Pb, the shoots, roots, flowers, and Pb-treated seedlings ofI. halophilawere subjected to single-molecular real-time sequencing and next-generation sequencing. A total of 40,034 complete coding sequences, 2028 alternative splices, 4649 long non-coding RNAs (lncRNAs) were predicted. Among them, 2614 annotated differentially expressed genes (DEGs) and 19 differentially expressed lncRNAs (DELs) were identified in Pb-treatedI. halophilashoot, while 7242 annotated DEGs and 57 DELs were identified in root under Pb conditions. Biological function enrichment analysis of DEGs revealed the key roles of hormone signaling transduction, GSH metabolism, metal ion transporters inI. halophilaresponse to Pb. LncRNA play crucial roles inI. halophilaresistance to Pb by regulating the key downstream metabolic pathways. These results not only enrich the genetic information ofIris. L plants, but also enhance the understanding of molecular responses to Pb inI. halophila, and will lay an important foundation for future research on Pb phytoremediation or low-Pb food crop and development ofI. halophilafor industrial uses.
登录
查看更多内容
DOI：
10.1016/j.bbrc.2011.09.030
发表时间：
2011-10
影响因子：
3.1
作者：
Xinxin Zhang;M. Zhang;T. Takano;Shenkui Liu
通讯作者：
Xinxin Zhang;M. Zhang;T. Takano;Shenkui Liu
两个高羊茅 (Festuca arundinacea) 品种对铅 (Pb) 胁迫反应的转录组分析
影响因子：
4.4
作者：
Li H;Hu T;Amombo E;Fu J
通讯作者：
Fu J
影响因子：
4.6
作者：
Yadav AK;Shankar A;Jha SK;Kanwar P;Pandey A;Pandey GK
通讯作者：
Pandey GK
影响因子：
13.6
作者：
Xiaoying Xu;Qi Chen;Shuangrong Mo;Ying Qian;Xiaoxia Wu;Yiqiao Jin;H. Ding
通讯作者：
Xiaoying Xu;Qi Chen;Shuangrong Mo;Ying Qian;Xiaoxia Wu;Yiqiao Jin;H. Ding
DOI：
10.1007/s00128-014-1444-x
发表时间：
2015-02-01
影响因子：
2.7
作者：
Gu, Chun-Sun;Liu, Liang-Qin;Lu, Xiao-Qing
通讯作者：
Lu, Xiao-Qing