Epigenetic clock and methylation studies in marsupials: opossums, Tasmanian devils, kangaroos, and wallabies.
Epigenetic clock and methylation studies in marsupials: opossums, Tasmanian devils, kangaroos, and wallabies.
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DOI：
10.1007/s11357-022-00569-5
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发表时间：
2022-06
期刊：
影响因子：
5.6
通讯作者：
Sears, Karen E.
中科院分区：
文献类型：
--
作者：
Horvath, Steve;Haghani, Amin;Zoller, Joseph A.;Raj, Ken;Sinha, Ishani;Robeck, Todd R.;Black, Pete;Couzens, Aidan;Lau, Clive;Manoyan, Meghety;Aviles Ruiz, Yadiamaris;Talbott, Annais;Belov, Katherine;Hogg, Carolyn J.;Sears, Karen E.
负鼠（Monodelphis arctica），其基因组测序，易于实验室护理和实验操作，以及独特的生物学，是最常用的实验室有袋动物。使用哺乳动物甲基化阵列，我们从n = 100负鼠样品的耳朵，肝脏和尾巴产生DNA甲基化数据。我们对比了出生后的发展和后期衰老的影响，负鼠甲基化与小鼠（小家鼠，C57 BL/6 J株）和其他有袋动物物种，如袋獾，袋鼠，和小袋鼠。虽然负鼠的甲基化组与小鼠在出生后发育过程中的甲基化组相似，但当涉及到多梳抑制复合物2靶位点甲基化的年龄相关增益时，它与其他哺乳动物所共有的甲基化组不同。我们的免疫组化染色结果提供了额外的支持，PRC 2活性增加，在小鼠组织中的老化，但在负鼠组织中保持不变的假设。我们提出了几个负鼠表观遗传时钟，其组织类型（泛组织和血液时钟）和物种（负鼠和人类）的兼容性区分。两个双物种人类负鼠泛组织时钟准确地测量实足年龄和相对年龄，分别。人类负鼠表观遗传时钟有望为负鼠作为生物模型的吸引力提供显着的提升。塔斯马尼亚魔鬼、红袋鼠和其他Macropus属物种的额外表观遗传时钟可能有助于物种保护工作。在线版本包含补充材料，可通过10.1007/s11357-022-00569-5获得。
The opossum (Monodelphis domestica), with its sequenced genome, ease of laboratory care and experimental manipulation, and unique biology, is the most used laboratory marsupial. Using the mammalian methylation array, we generated DNA methylation data from n = 100 opossum samples from the ear, liver, and tail. We contrasted postnatal development and later aging effects in the opossum methylome with those in mouse (Mus musculus, C57BL/6 J strain) and other marsupial species such as Tasmanian devil, kangaroos, and wallabies. While the opossum methylome is similar to that of mouse during postnatal development, it is distinct from that shared by other mammals when it comes to the age-related gain of methylation at target sites of polycomb repressive complex 2. Our immunohistochemical staining results provide additional support for the hypothesis that PRC2 activity increases with later aging in mouse tissues but remains constant in opossum tissues. We present several epigenetic clocks for opossums that are distinguished by their compatibility with tissue type (pan-tissue and blood clock) and species (opossum and human). Two dual-species human-opossum pan-tissue clocks accurately measure chronological age and relative age, respectively. The human-opossum epigenetic clocks are expected to provide a significant boost to the attractiveness of opossum as a biological model. Additional epigenetic clocks for Tasmanian devil, red kangaroos and other species of the genus Macropus may aid species conservation efforts. The online version contains supplementary material available at 10.1007/s11357-022-00569-5.
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CpG岛密度及其与哺乳动物基因组中基因组特征的相关性。
影响因子：
12.3
作者：
Han L;Su B;Li WH;Zhao Z
通讯作者：
Zhao Z
影响因子：
3.2
作者：
Cordeiro, Matheus Dias;Baeta, Bruna de Azevedo;da Fonseca, Adivaldo Henrique
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da Fonseca, Adivaldo Henrique
影响因子：
2.4
作者：
Dowling, Anna;Doroba, Carolyn;Sears, Karen E.
通讯作者：
Sears, Karen E.
影响因子：
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作者：
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通讯作者：
Krubitzer, Leah A.
哺乳动物甲基化阵列，用于在保守序列上分析甲基化水平。
影响因子：
16.6
作者：
Arneson A;Haghani A;Thompson MJ;Pellegrini M;Kwon SB;Vu H;Maciejewski E;Yao M;Li CZ;Lu AT;Morselli M;Rubbi L;Barnes B;Hansen KD;Zhou W;Breeze CE;Ernst J;Horvath S
通讯作者：
Horvath S