Substrate specificity and safener inducibility of the plant UDP-glucose-dependent family 1 glycosyltransferase super-family.
Substrate specificity and safener inducibility of the plant UDP-glucose-dependent family 1 glycosyltransferase super-family.
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植物UDP-葡萄糖依赖性家族1糖基转移酶超级家族的底物特异性和保险剂诱导性。
DOI：
10.1111/pbi.12775
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发表时间：
2018-01
影响因子：
13.8
通讯作者：
Edwards R
中科院分区：
文献类型：
--
作者：
Brazier-Hicks M;Gershater M;Dixon D;Edwards R
植物含有大量的家族1 UDP-葡萄糖依赖性糖基转移酶（UGT），包括缀合异生物质的成员。拟南芥含有107个UGT基因，其中99个家族成员在大肠杆菌中成功表达为谷胱甘肽转移酶（GST）融合蛋白。基于通过测量GST活性对融合物进行定量，开发了高通量催化筛选。使用UDP-葡萄糖作为供体的UGT活性然后使用11种带有羟基、氨基和巯基的合成受体测定，这些受体已显示在植物提取物中经历缀合。总共有44个UGT，主要是D和E组的成员，对外源性物质、葡糖基化苯酚和巯基受体有活性。相比之下，N-葡糖基转移酶（NGT）活性几乎仅限于单一酶UGT 72 B1。使用DNA微阵列，诱导UGT转录后的除草剂安全剂fenclorim处理在拟南芥和水稻进行了比较。D和L组成员是两个物种中最安全的诱导型UGT。相应的拟南芥酶显示出对异生物质的低缀合活性。使用Genevestigator，一小组的safened D和L UGT一致诱导的生物和非生物胁迫暗示的保护活动超出了两个物种的异生物质解毒。其他解毒基因家族的诱导与fenclorim，即细胞色素P450和谷胱甘肽转移酶处理后，进一步证实了相关的亚家族成员在两个物种中的选择性增强提供了新的见解，在谷物中的安全化反应，其中除草剂耐受性增强与双子叶植物相比，这是不响应这些治疗。
Plants contain large numbers of family 1 UDP‐glucose‐dependent glycosyltransferases (UGTs), including members that conjugate xenobiotics. Arabidopsis contains 107 UGT genes with 99 family members successfully expressed as glutathione transferase (GST)‐fusion proteins in E. coli. A high‐throughput catalytic screen was developed based on quantification of the fusion by measuring GST activity. UGT activity using UDP‐glucose as donor was then determined using 11 synthetic acceptors bearing hydroxyl, amino and thiol groups that had been shown to undergo conjugation in plant extracts. In total, 44 UGTs, largely members of the D and E groups, were active towards xenobiotics, glucosylating phenol and thiol acceptors. In contrast, N‐glucosyltransferase (NGT) activity was almost exclusively restricted to a single enzyme, UGT72B1. Using DNA microarrays, the induction of UGT transcripts following treatment with the herbicide safener fenclorim was compared in Arabidopsis and rice. D and L group members were the most safener‐inducible UGTs in both species. The respective Arabidopsis enzymes showed low conjugating activity towards xenobiotics. Using Genevestigator, a small group of safened D and L UGTs were consistently induced in response to biotic and abiotic stress suggestive of protective activities beyond xenobiotic detoxification in both species. The induction of other detoxifying gene families following treatment with fenclorim, namely cytochromes P450 and glutathione transferases, further confirmed the selective enhancement of related subfamily members in the two species giving new insight into the safening response in cereals, where herbicide tolerance is enhanced compared with dicots, which are unresponsive to these treatments.
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影响因子：
3.7
作者：
Duhoux A;Délye C
通讯作者：
Délye C
影响因子：
7.4
作者：
Iwakami, Satoshi;Endo, Masaki;Inamura, Tatsuya
通讯作者：
Inamura, Tatsuya
影响因子：
7.2
作者：
Brazier-Hicks, M;Edwards, R
通讯作者：
Edwards, R
影响因子：
4.3
作者：
Lim, EK;Baldauf, S;Bowles, DJ
通讯作者：
Bowles, DJ
DOI：
10.1073/pnas.0706421104
发表时间：
2007-12-18
影响因子：
11.1
作者：
Brazier-Hicks, Melissa;Offen, Wendy A.;Edwards, Robert
通讯作者：
Edwards, Robert