Reduced numbers of T cells and B cells correlates with persistent SARS-CoV-2 presence in non-severe COVID-19 patients.
Reduced numbers of T cells and B cells correlates with persistent SARS-CoV-2 presence in non-severe COVID-19 patients.
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DOI：
10.1038/s41598-020-73955-8
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发表时间：
2020-10-19
影响因子：
4.6
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Liu B;Han J;Cheng X;Yu L;Zhang L;Wang W;Ni L;Wei C;Huang Y;Cheng Z
COVID-19已经广泛传播。我们的目的是检查持续SARS-CoV-2脱落的非重症患者的适应性免疫细胞。方法：回顾性分析武汉大学中南医院收治的37例SARS冠状病毒2型（SARS-CoV-2）持续存在的非重症患者，将其纳入PP组（持续阳性），根据7 d后检测结果分为PPP组（n = 19）和PPN组（n = 18）（N =阴性）。收集并分析流行病学、人口统计学、临床和实验室数据。从疾病发作时年龄和性别匹配的非重度患者（PA [入院时阳性]患者，n = 37）中提取数据，并从匹配的54名健康受试者中提取淋巴细胞亚群测量值进行比较（HC）。与PA患者相比，PP患者的实验室检查结果明显改善。PP组CD 3 + T细胞、CD 4 + T细胞和NK细胞绝对数均显著高于PA组，与健康对照组无显著性差异。PPP亚组的B细胞和T细胞较PPN组和健康对照组明显减少。最后，来自10名PPN患者的这些淋巴细胞亚群的配对结果表明，当SARS-CoV-2检测转为阴性时，T细胞和B细胞的数量显著增加。非重症COVID-19患者持续存在SARS-CoV-2与适应性免疫细胞数量减少有关。监测淋巴细胞亚群可能对识别完全康复的COVID-19患者具有临床意义。
COVID-19 has been widely spreading. We aimed to examine adaptive immune cells in non-severe patients with persistent SARS-CoV-2 shedding. 37 non-severe patients with persistent SARS-CoV-2 presence that were transferred to Zhongnan hospital of Wuhan University were retrospectively recruited to the PP (persistently positive) group, which was further allocated to PPP group (n = 19) and PPN group (n = 18), according to their testing results after 7 days (N = negative). Epidemiological, demographic, clinical and laboratory data were collected and analyzed. Data from age- and sex-matched non-severe patients at disease onset (PA [positive on admission] patients, n = 37), and lymphocyte subpopulation measurements from matched 54 healthy subjects were extracted for comparison (HC). Compared with PA patients, PP patients had much improved laboratory findings. The absolute numbers of CD3+ T cells, CD4+ T cells, and NK cells were significantly higher in PP group than that in PA group, and were comparable to that in healthy controls. PPP subgroup had markedly reduced B cells and T cells compared to PPN group and healthy subjects. Finally, paired results of these lymphocyte subpopulations from 10 PPN patients demonstrated that the number of T cells and B cells significantly increased when the SARS-CoV-2 tests turned negative. Persistent SARS-CoV-2 presence in non-severe COVID-19 patients is associated with reduced numbers of adaptive immune cells. Monitoring lymphocyte subpopulations could be clinically meaningful in identifying fully recovered COVID-19 patients.
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影响因子：
1.8
作者：
Lee HK;Lee BH;Seok SH;Baek MW;Lee HY;Kim DJ;Na YR;Noh KJ;Park SH;Kumar DN;Kariwa H;Nakauchi M;Heo SJ;Park JH
通讯作者：
Park JH
影响因子：
11.8
作者：
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影响因子：
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作者：
Li, Zhengtu;Yi, Yongxiang;Ye, Feng
通讯作者：
Ye, Feng
DOI：
10.1016/j.jaip.2020.06.015
发表时间：
2020-09-01
影响因子：
9.4
作者：
Chang, De;Zhao, Peng;Qin, En-Qiang
通讯作者：
Qin, En-Qiang
DOI：
10.1016/s0140-6736(04)16255-7
发表时间：
2004-05-22
期刊：
Lancet (London, England)
影响因子：
--
作者：
Cheng PK;Wong DA;Tong LK;Ip SM;Lo AC;Lau CS;Yeung EY;Lim WW
通讯作者：
Lim WW