Combined treatment with FK506 and nerve growth factor for spinal cord injury in rats.
Combined treatment with FK506 and nerve growth factor for spinal cord injury in rats.
复制标题
FK506联合神经生长因子治疗大鼠脊髓损伤
DOI：
10.3892/etm.2013.1254
复制
发表时间：
2013-10
影响因子：
2.7
通讯作者：
Lv D
中科院分区：
文献类型：
--
作者：
Chen G;Zhang Z;Wang S;Lv D
大鼠脊髓损伤后，FK506具有保护局部神经组织，促进神经再生，减少神经元凋亡，加速脊髓功能恢复的作用。神经生长因子（NGF）在中枢和周围神经细胞的再生、生长分化和功能调控中具有重要作用。既往研究表明，FK506和NGF在治疗周围神经损伤中表现出协同作用；然而，在脊髓损伤的治疗中是否存在协同效应尚不清楚。本研究将FK506与NGF联合治疗大鼠脊髓损伤。免疫组化染色法检测脊髓损伤大鼠NF200蛋白表达，逆转录-聚合酶链反应法观察NF200 mRNA表达水平。使用Basso， Beattie和Bresnahan评分评估脊髓功能的恢复情况。结果表明，与单独治疗相比，联合治疗显著提高了NF200的表达，改善了脊髓功能。我们的实验观察表明，FK506和NGF在大鼠脊髓损伤治疗中表现出协同作用，联合治疗可有效促进脊髓损伤后大鼠神经再生和功能恢复。
Following spinal cord injury in rats, FK506 is able to protect local nerve tissue, promote neural regeneration, reduce neuronal apoptosis and accelerate the recovery of spinal cord functions. Nerve growth factor (NGF) is important in the regulation of central and peripheral nerve cell regeneration, growth differentiation and functions. Previous studies have shown that FK506 and NGF exhibit a synergistic effect in the treatment of peripheral nerve injury; however, it remains unclear whether the synergistic effect is present in the treatment of spinal cord injury. In this study, we combined FK506 and NGF for the treatment of spinal cord injury in rats. The NF200 protein expression in rats with spinal cord injury was determined using immunohistochemical staining and NF200 mRNA expression levels were observed using the reverse transcription-polymerase chain reaction method. The restoration of spinal cord functions was evaluated using the Basso, Beattie and Bresnahan score. The results demonstrated that the combined treatment significantly enhanced the expression of NF200 and improved spinal cord functions compared with the results of the single treatment. Our experimental observations indicated that FK506 and NGF exhibit a synergistic effect in the treatment of spinal cord injury in rats and that the combined treatment may effectively promote neural regeneration and functional recovery in rats following spinal cord injury.
登录
查看更多内容
影响因子：
4.8
作者：
Asai, A;Qiu, JH;Kirino, T
通讯作者：
Kirino, T
影响因子：
1.7
作者：
Kim, DH;Gutin, PH;Nockels, RP
通讯作者：
Nockels, RP
影响因子：
4.2
作者：
López-Vales, R;García-Alías, G;Verdú, E
通讯作者：
Verdú, E
影响因子：
7.3
作者：
Price, RD;Yamaji, T;Matsuoka, N
通讯作者：
Matsuoka, N
影响因子：
5.3
作者：
Nottingham, S;Knapp, P;Springer, J
通讯作者：
Springer, J