Chromosome assignments of rat phenol sulfotransferase ST1A1 and ST1C1 genes (Sult1a1 and Sult1c1) by fluorescence in situ hybridization
Chromosome assignments of rat phenol sulfotransferase ST1A1 and ST1C1 genes (Sult1a1 and Sult1c1) by fluorescence in situ hybridization
复制标题
通过荧光原位杂交对大鼠苯酚磺基转移酶 ST1A1 和 ST1C1 基因（Sult1a1 和 Sult1c1）进行染色体分配
DOI：
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发表时间：
1999
影响因子：
1.7
通讯作者：
M. Matsui
中科院分区：
文献类型：
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作者：
F. Nagai;H. Satoh;T. Usui;H. Tamura;M. Matsui
硫转移酶（ST）在内源性和外源性化合物的生物转化和解毒中起重要作用，是一个多基因家族。在哺乳动物中，STs被分为羟基类固醇ST和酚ST家族（Weinshilboum et al., 1997）。苯酚ST家族进一步细分为苯酚ST和雌激素ST亚家族。在之前的一篇论文中，我们通过荧光原位杂交将两个大鼠羟类固醇ST基因Sth1和Sth2定位到染色体1q21.3→q22.1的同一区域（Nagai et al., 1996）。在本研究中，我们确定了两个大鼠酚STs的基因位点。大鼠苯酚ST， ST1A1 (Sult1a1) cDNA编码291个氨基酸，表达的酶催化苯酚，儿茶酚胺和米诺地尔的硫酸化（Ozawa et al., 1993）。另一种大鼠苯酚ST， ST1C1 (Sult1c1) cDNA编码304个氨基酸，表达的酶对酚类和一种强致癌物n羟基-2-乙酰氨基荧光素具有高ST活性（Nagata et al., 1993）。这些cdna的氨基酸序列有51%相同。为了确定它们的染色体位置，我们利用PCR制备的生物素化cDNA探针进行了荧光原位杂交。材料与方法
Sulfotransferase (ST) enzymes play an important role in the biotransformation and detoxification of endogenous and xenobiotic compounds, and comprise a multigene family. In mammals, STs are classified into hydroxysteroid ST and phenol ST families (Weinshilboum et al., 1997). The phenol ST family is further subdivided into phenol ST and estrogen ST subfamilies. In a previous paper, we assigned two rat hydroxysteroid ST genes, Sth1 and Sth2, to the same chromosome region of 1q21.3→q22.1 by fluorescence in situ hybridization (Nagai et al., 1996). In the present study, we determined the gene loci of two rat phenol STs. Rat phenol ST, ST1A1 (Sult1a1) cDNA encodes 291 amino acids and the expressed enzyme catalyzes sulfation of phenols, catecholamines and minoxidil (Ozawa et al., 1993). Another rat phenol ST, ST1C1 (Sult1c1) cDNA encodes 304 amino acids and the expressed enzyme shows high ST activity toward phenols and a potent carcinogen, Nhydroxy-2-acetylaminofluorene (Nagata et al., 1993). These cDNAs are 51% identical in amino acid sequence. In order to determine their chromosome locations, we carried out fluorescence in situ hybridization using biotinylated cDNA probes, which were prepared by PCR. Materials and methods
将苯酚磺基转移酶基因 (STP) 映射到人类染色体 16p12.1-p11.2 和小鼠染色体 7。
影响因子：
4.4
作者：
Dooley,TP;Obermoeller,RD;Leiter,EH;Chapman,HD;Falany,CN;Deng,Z;Siciliano,MJ
通讯作者：
Siciliano,MJ