Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine.
Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine.
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DOI：
10.1002/advs.202302165
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发表时间：
2024-02
期刊：
影响因子：
--
通讯作者：
Gentleman E
中科院分区：
文献类型：
--
作者：
Cameron O;Neves JF;Gentleman E
肠道通过分解食物和吸收营养物质，同时保持对肠道微生物组的选择性屏障来执行对人类健康至关重要的功能。这种屏障功能的关键是腔衬特化肠上皮细胞和支持性的基础免疫细胞丰富的基质组织的共同努力。肠上皮细胞可以在体外复制为肠类器官的发现为了解肠道发育，稳态和疾病提供了一种新的方法。然而，类器官反映了孤立的肠上皮，而底层组织也包含无数的细胞类型以及令人印象深刻的化学和结构复杂性。这篇综述剖析了肠道的细胞和基质成分，并讨论了使用自下而上的生物自组织和自上而下的生物工程原理在体外复制它们的策略。它还涵盖了肠道微环境的细胞，生物化学和生物物理特征，以及如何通过将类器官生物学与材料科学相结合的策略在体外复制这些特征。特别是可访问的化学物质，模仿天然细胞外基质进行了讨论，和生物工程的方法，旨在克服局限性，在模拟肠道进行了严格的评估。最后，审查考虑如何进一步的进展可能会扩大肠模型的应用和其适用于临床治疗。肠上皮细胞可以在体外复制为肠类器官;然而，天然肠还包含无数的基质细胞类型和令人印象深刻的生化和结构复杂性。这篇综述剖析了肠道的细胞和物理成分，并讨论了在体外复制它们的生物工程方法，以创建用于疾病建模和再生治疗的先进肠道模型。
The intestine performs functions central to human health by breaking down food and absorbing nutrients while maintaining a selective barrier against the intestinal microbiome. Key to this barrier function are the combined efforts of lumen‐lining specialized intestinal epithelial cells, and the supportive underlying immune cell‐rich stromal tissue. The discovery that the intestinal epithelium can be reproduced in vitro as intestinal organoids introduced a new way to understand intestinal development, homeostasis, and disease. However, organoids reflect the intestinal epithelium in isolation whereas the underlying tissue also contains myriad cell types and impressive chemical and structural complexity. This review dissects the cellular and matrix components of the intestine and discusses strategies to replicate them in vitro using principles drawing from bottom‐up biological self‐organization and top‐down bioengineering. It also covers the cellular, biochemical and biophysical features of the intestinal microenvironment and how these can be replicated in vitro by combining strategies from organoid biology with materials science. Particularly accessible chemistries that mimic the native extracellular matrix are discussed, and bioengineering approaches that aim to overcome limitations in modelling the intestine are critically evaluated. Finally, the review considers how further advances may extend the applications of intestinal models and their suitability for clinical therapies. The intestinal epithelium can be reproduced in vitro as intestinal organoids; however, the native intestine also contains myriad stromal cell types and impressive biochemical and structural complexity. This review dissects the cellular and physical components of the intestine and discusses bioengineering approaches for replicating them in vitro to create advanced models of the intestine for disease modelling and regenerative therapies.
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影响因子：
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作者：
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作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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