Identification of Conserved and Divergent Strigolactone Receptors in Sugarcane Reveals a Key Residue Crucial for Plant Branching Control.
Identification of Conserved and Divergent Strigolactone Receptors in Sugarcane Reveals a Key Residue Crucial for Plant Branching Control.
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DOI：
10.3389/fpls.2021.747160
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发表时间：
2021
影响因子：
5.6
通讯作者：
Yao R
中科院分区：
文献类型：
--
作者：
Hu A;Zhao Q;Chen L;Zhao J;Wang Y;Feng K;Wu L;Xie M;Zhou X;Xiao L;Ming Z;Zhang M;Yao R
独脚金内酯（Strigolactones，SL）是一类重要的植物激素，主要调节植物分枝等结构，对作物产量起着重要作用。甘蔗是重要的糖料作物，深入研究SL信号通路及其生理功能对进一步开展甘蔗分子育种具有重要意义。在这里，两个推定的SL受体SsD 14 a/B和相互作用的F盒蛋白SsMAX 2被确定在密割密茎中。在酵母中，SL诱导SsD 14 a和SsD 14 b与SsMAX 2相互作用。SsD 14 a能与AtMAX 2和AtSMXL 7/SsSMXL 7结合，而SsD 14 b不能。SsD 14 a或SsMAX 2的过表达分别挽救了拟南芥d14-1或max2-3突变体的增加的分支表型。此外，解析了N端截短的SsD 14 a的晶体结构，其在开放状态下的整体结构与AtD 14和OsD 14相同，与其在拟南芥中保守的分支抑制能力一致。与生化观察结果一致，SsD 14 b在d14-1中不能完全互补，尽管这两种SsD 14蛋白除了极少数残基外具有几乎相同的一级序列。用SsD 14 b和SsMAX 2的组合进行互补仍然未能拯救d14-1 max2-3双突变体多分支表型，表明SsD 14 b-AtSMXL 7复合物形成是调节分支所需的。突变分析表明，SsD 14 a α10螺旋上的R310残基是与SsSMXL 7/AtSMXL 7结合的关键，而与SsMAX 2结合不明显。位点等价的单残基P304 R取代使SsD 14 b能够与AtMAX 2和AtSMXL 7/SsSMXL 7结合，并与SsMAX 2一起拯救d14-1 max 2 -3的表型。此外，这种保守的Arg残基跨物种，包括水稻和拟南芥决定SL受体的活性，通过维持它们与SMXL阻遏物的相互作用。综上所述，我们的工作确定了甘蔗核心SL信号通路中保守和不同的独脚金内酯受体，并揭示了植物分枝控制的关键残基。
Strigolactones (SLs) are a class of important plant hormones mainly regulating plant architecture such as branching, which is crucial for crop yield. It is valuable to study SL signaling pathway and its physiological function in sugarcane, the most important sugar crop, for further molecular breeding. Here, two putative SL receptors SsD14a/b and the interacting F-box protein SsMAX2 were identified in Saccharum spontaneum. SL induced both SsD14a and SsD14b to interact with SsMAX2 in yeast. SsD14a, but not SsD14b, could bind with AtMAX2 and AtSMXL7/SsSMXL7. Overexpression of SsD14a or SsMAX2 rescued the increased branching phenotypes of Arabidopsis thaliana d14-1 or max2-3 mutants, respectively. Moreover, the crystal structure of N-terminal truncated SsD14a was solved, with an overall structure identical to AtD14 and OsD14 in the open state, consistent with its conserved branching suppression capacity in Arabidopsis. In line with the biochemical observations, SsD14b could not completely complement in d14-1 although these two SsD14 proteins have almost identical primary sequences except for very few residues. Complement with the combination of SsD14b and SsMAX2 still failed to rescue the d14-1 max2-3 double mutant multi-branching phenotype, indicating SsD14b–AtSMXL7 complex formation is required for regulating branching. Mutagenesis analyses revealed that residue R310 at α10 helix of SsD14a was crucial for the binding with SsSMXL7/AtSMXL7 but not SsMAX2. The site-equivalent single-residue P304R substitution enabled SsD14b to bind with AtMAX2 and AtSMXL7/SsSMXL7 and to rescue the phenotype of d14-1 max2-3 together with SsMAX2. Moreover, this conserved Arg residue across species including rice and Arabidopsis determined the activity of SL receptors through maintaining their interaction with SMXL repressors. Taken together, our work identified conserved and divergent strigolactone receptors in sugarcane core SL signaling pathway and revealed a key residue crucial for plant branching control.
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