Expression patterns of tight junction proteins in porcine major salivary glands: a comparison study with human and murine glands
Expression patterns of tight junction proteins in porcine major salivary glands: a comparison study with human and murine glands
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猪大唾液腺紧密连接蛋白的表达模式：与人类和小鼠腺体的比较研究
DOI：
10.1111/joa.12833
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Yan Zhang
中科院分区：
文献类型：
--
作者：
Xue-Ming Zhang;Yan Huang;Kuo Zhang;Ling-Han Qu;Xin Cong;Jia-Zeng Su;Li-Ling Wu;Guang-Yan Yu;Yan Zhang
紧密连接（TJ）蛋白在唾液腺上皮细胞旁细胞液体运输中发挥着动态作用。大多数 TJ 研究都是在小鼠和大鼠中进行的。然而，啮齿动物唾液腺的形态与人类腺体不同。本研究旨在比较猪唾液腺与人和小鼠唾液腺中 TJ 蛋白的组织学特征和表达模式。结果表明，猪腮腺为纯浆液性腺。颌下腺（SMG）是以浆液性腺泡细胞为主的混合腺体，而舌下腺是以粘液性腺泡细胞为主的混合腺体。人类 SMG 是混合腺体，含有比猪 SMG 更少的粘液细胞，而鼠 SMG 的腺泡细胞是浆液粘液的。猪和人 SMG 的导管系统的组织学特征相似，包括闰管、横纹管和排泄管，但鼠 SMG 包含一种特殊的结构，即颗粒曲小管。通过 RT-PCR 在猪大唾液腺和人 SMG 中检测到 TJ 蛋白，包括claudin-1至claudin-12、occludin和小带occludin-1 (ZO-1)；然而，在小鼠 SMG 中未检测到claudin-6、claudin-9 和claudin-11。免疫荧光显示，猪和人SMG中claudin-1、claudin-3、claudin-4、occludin和ZO-1均分布于腺泡和导管细胞中，而鼠SMG中claudin-1和claudin-3主要分布于腺泡细胞中，claudin-4主要分布于导管细胞中。此外，3D 图像显示，TJ 蛋白在导管管腔表面上呈蜂窝状结构排列，而在猪 SMG 中，它们在腺泡中的排列是不规则的。综上所述，人和小型猪唾液腺中TJ蛋白的表达模式相似，可能是研究唾液腺TJ功能的候选动物。
Tight junction (TJ) proteins play a dynamic role in paracellular fluid transport in salivary gland epithelia. Most TJ studies are carried out in mice and rats. However, the morphology of rodent salivary glands differs from that of human glands. This study aimed to compare the histological features and the expression pattern of TJ proteins in porcine salivary glands with those of human and mouse. The results showed that porcine parotid glands were pure serous glands. Submandibular glands (SMGs) were serous acinar cell‐predominated mixed glands, whereas sublingual glands were mucous acinar cell‐predominated. Human SMGs were mixed glands containing fewer mucous cells than porcine SMGs, whereas the acinar cells of murine SMGs are seromucous. The histological features of the duct system in the porcine and human SMGs were similar and included intercalated, striated and excretory ducts, but the murine SMG contained a specific structure, the granular convoluted tubule. TJ proteins, including claudin‐1 to claudin‐12, occludin and zonula occludin‐1 (ZO‐1), were detected in the porcine major salivary glands and human SMGs by RT‐PCR; however, claudin‐6, claudin‐9 and claudin‐11 were not detected in the murine SMG. As shown by immunofluorescence, claudin‐1, claudin‐3, claudin‐4, occludin and ZO‐1 were distributed in both acinar and ductal cells in the porcine and human SMGs, whereas claudin‐1 and claudin‐3 were mainly present in acinar cells, and claudin‐4 was mainly distributed in ductal cells in the murine SMG. In addition, 3D images showed that the TJ proteins arranged in a honeycomb‐like structure on the luminal surface of the ducts, whereas their arrangements in acini were irregular in porcine SMGs. In summary, the expression pattern of TJ proteins in salivary glands is similar between human and miniature pig, which may be a candidate animal for studies on salivary gland TJ function.
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影响因子：
3.2
作者：
Nam, Kihoon;Maruyama, Christina L.;Baker, Olga J.
通讯作者：
Baker, Olga J.
DOI：
10.1002/aja.1001110104
发表时间：
1962-07
期刊：
The American journal of anatomy
影响因子：
--
作者：
J. Shackleford;C. Klapper
通讯作者：
J. Shackleford;C. Klapper
影响因子：
9.8
作者：
Müller, D;Kausalya, PJ;Hunziker, W
通讯作者：
Hunziker, W
影响因子：
5.1
作者：
Guo, L.;Gao, R.;Wang, S.
通讯作者：
Wang, S.
影响因子：
5.1
作者：
Wang Z;Zourelias L;Wu C;Edwards PC;Trombetta M;Passineau MJ
通讯作者：
Passineau MJ